|CHEN, JING - Oak Ridge Institute For Science And Education (ORISE)|
Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 12/13/2017
Publication Date: 3/18/2018
Citation: Chen, J., Park, B. 2018. Multiplex and label-free screening of foodborne pathogens using surface plasmon resonance imaging. American Chemical Society National Meeting.
Interpretive Summary: n/a
Technical Abstract: In order to protect outbreaks caused by foodborne pathogens, more rapid and efficient methods are needed for pathogen screening from food samples. Surface plasmon resonance imaging (SPRi) is an emerging optical technique, which allows for label-free screening of multiple targets simultaneously with minimum or no sample preparation. In this study, we have evaluated the feasibility of SPRi in simultaneous detection of four most important foodborne pathogens, Salmonella spp., Shiga-toxin producing E. coli, Listeria monocytogenes, and Campylobacter jejuni. The SPRi-biochip was functionalized with corresponding polyclonal antibodies and blocking agents. Bacterial cells were tested for performance of the multiplex SPRi method. The influence of antibody concentration and immobilization pH were optimized, and the specificity was evaluated. The cells were also fragmentized thermally and ultrasonically, whose SPRi signals were compared with intact cells. Overall, the new SPRi technique demonstrated the potentials in multiplex pathogen screening, and with further improvement in sensitivity, this platform could provide a flexible and automated means for pathogen detection in food matrices.