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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics, Breeding, and Animal Health Research » Research » Publications at this Location » Publication #349848

Research Project: Genomic Intervention Strategies to Prevent and/or Treat Respiratory Diseases of Ruminants

Location: Genetics, Breeding, and Animal Health Research

Title: Genomic signatures of a Mannheimia haemolytica lineage associated with bovine respiratory disease

Author
item Clawson, Michael - Mike
item Schuller, Genevieve - Gennie
item Dickey, Aaron
item Murray, Robert - Zoetis
item Sweeney, Michael - Zoetis
item Apley, Michael - Kansas State University
item Dedonder, Keith - Veterinary And Biomedical Research Center, Inc
item Capik, Sarah - Texas A&M Agrilife
item Larson, Robert - Kansas State University
item Lubbers, Brian - Kansas State University
item White, Brad - Kansas State University
item Blom, Jochen - Justus-Liebig University
item Harhay, Dayna
item Smith, Timothy - Tim

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: A major challenge in the modern age of microbiology is to develop narrow-spectrum interventions that target pathogens, but not the remaining microbiome of a host, including less- or non-virulent strains of the same pathogenic species. Mannheimia haemolytica is a model study organism in that regard. M. haemolytica is both a normal resident of the upper respiratory tract of cattle, and a major cause of bovine respiratory disease. Sequencing of over 1,100 isolate genomes has shown that there are two major lineages, or genotypes of M. haemolytica (1 and 2) in North American cattle. While both genotypes are found in the upper and lower respiratory tract of cattle with or without signs of disease, genotype 2 predominantly associates with the diseased lungs of cattle over genotype 1. Over 13,000 genome-wide polymorphism alleles separate the two lineages. Additionally, an integrative conjugative element with variable numbers of antimicrobial resistance genes has been found in all genotype 2 isolates examined to date, and rarely in genotype 1 isolates. The two genotypes share a core genome of 1,880 proteins, and differ by 112 and 179 proteins with specificity for genotype 1 and 2, respectively. Analyses of the proteins specific to each genotype, and those shared in the core, particularly those with beta-barrel topology which is indicative of outer membrane localization in Gram negative bacteria, point towards the design of next-generation vaccines that could target only genotype 2 M. haemolytica to minimize disruption to the bovine microbiome, or both genotypes if the need arises.