Skip to main content
ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Genetics, Breeding, and Animal Health Research » Research » Publications at this Location » Publication #337187

Research Project: Genomic Intervention Strategies to Prevent and/or Treat Respiratory Diseases of Ruminants

Location: Genetics, Breeding, and Animal Health Research

Title: Closed genomes and phenotypes of seven Histophilus somni isolates from beef calves with bovine respiratory disease complex

Author
item Harhay, Gregory
item Harhay, Dayna
item Bono, James - Jim
item Smith, Timothy - Tim
item Heaton, Michael - Mike
item Clawson, Michael - Mike
item Chitko Mckown, Carol
item Capik, Sarah - Texas A&M Agricultural Experiment Station
item Dedonder, Keith - Veterinary And Biomedical Research Center, Inc
item Apley, Michael - Kansas State University
item Lubbers, Brian - Kansas State University
item White, Bradley - Kansas State University
item Larson, Robert - Kansas State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 2/27/2017
Publication Date: 6/4/2017
Citation: Harhay, G.P., Harhay, D.M., Bono, J.L., Smith, T.P., Heaton, M.P., Clawson, M.L., Chitko-McKown, C.G., Capik, S.F., DeDonder, K.D., Apley, M.D., Lubbers, B.V., White, B.J., Larson, R.L. 2017. Closed genomes and phenotypes of seven Histophilus somni isolates from beef calves with bovine respiratory disease complex [Abstract]. American Society for Microbiology Microbe Meeting, June 1-5, 2017, New Orleans, LA. Poster #Sunday-869.

Interpretive Summary:

Technical Abstract: Background: Histophilus somni is a fastidious gram-negative opportunistic pathogenic Pasteurellacea that affects multiple organ systems and is one of three principle bacterial species contributing to bovine respiratory disease complex (BRDC) in North American feed yard cattle. BRDC outbreaks account for up to 75% of feed yard cattle morbidity and are the primary drivers of disease-related antibiotic treatments. BRDC is the costliest disease for most North American feed yard cattle. It is thought that a primary H. somni respiratory infection precedes a secondary bloodborne infection facilitating the spread to other organs. There is a need for more publicly available H. somni closed genomes because only two were available in GenBank as of January 2016. Goal: To identify pathogenicity and virulence determinants of H. somni through the analysis of genomic, epigenomic, and phenotypic data. Approach: Nasopharyngeal and bronchoalveolar lavage (BAL) isolates were cultured from animals exhibiting clinical signs of BRDC at a Kansas feed yard during a 28-day study of 180 calves originating from 3 different southeastern U.S. states. Closed genomes and methylomes of these isolates were obtained with single molecule real-time sequencing. Antibiotic resistance, metabolic, and liquid chromatography – mass spectrometry phenotypes of these isolates were collected and analyzed. Results: Four H. somni isolates (three nasopharyngeal, one BAL) were identified from a single case animal classified as an antibiotic treatment failure. Three H. somni isolates (one nasopharyngeal, two BAL) were identified from two additional case animals. The closed genomes of these seven H. somni isolates, their methylomes, and phenotypic profiles will be compared.