Location: Avian Disease and Oncology ResearchTitle: Transcriptional analyses of response to vaccines in two highly inbred lines of White Leghorns resistant or susceptible to Marek’s disease
|DONG, KUNZHE - Oak Ridge Institute For Science And Education (ORISE)|
|CHANG, SHUANG - Shandong Agricultural University|
|XIE, OINGMEI - South China Agricultural University|
Submitted to: International Marek's Disease Symposium Abstracts and Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/9/2016
Publication Date: 7/6/2016
Citation: Dong, K., Chang, S., Xie, O., Zhang, H. 2016. Transcriptional analyses of response to vaccines in two highly inbred lines of White Leghorns resistant or susceptible to Marek’s disease [abstract]. In: Proceeding for the 11th International Symposium on Marek's Disease and Avian Herpesviruses, July 6-9, 2016, Tours, France. p. 69.
Technical Abstract: The protective efficacy of Marek’s disease (MD) vaccines could significantly differ between some chicken lines. One of the factors that contribute to this variability is attributable to host genetics. This study was designed to identify genes that may potentially modulate vaccine protective efficacy. RNA-Seq analyses were performed to profile differentially expressed genes in response to MD vaccines at 5, 10, and 21-day post inoculation (dpi) in two highly inbred lines of chickens, lines 63 and 72. Both HVT and CVI988/Rispens convey good protection to line 63 birds, but HVT conveys minimal and CVI988/Rispens conveys relative poor protection to line 72. A total of 243, 325, and 70 differentially expressed genes, in contrast to the control counterparts, were identified in response to vaccination at the three time points, respectively. Gene enrichment analyses revealed that both vaccines significantly down-regulated defense response genes in line 63 at 5 dpi, while significantly down-regulated metabolism-related genes at 10 dpi in line 72. HVT elicited down-regulation of genes that are involved in NOD-like receptor signaling pathway and Cytokine-cytokine receptor interaction pathway in line 72 birds at 21 dpi, which may well lead to significant suppression of immune capacity in the birds. Our findings from this study suggest multiple genes may be involved in adaptive immunity and modulate biological processes in response to vaccination in preventing tumorigenesis. It is anticipated data from this study may well facilitate future investigations on genetic mechanisms underlying vaccine protective efficacy, which is of critical importance to both poultry and vaccine industries.