Location: Renewable Product Technology ResearchTitle: Production of polyol oils from soybean oil by bioprocess and Philippines edible medicinal wild mushrooms
|DULAY, RICH - Central Luzon State University|
Submitted to: American Oil Chemists' Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/2015
Publication Date: 11/15/2015
Citation: Hou, C.T., Lin, J.T., Dulay, R., Ray, K.J. 2015. Production of polyol oils from soybean oil by bioprocess and Philippines edible medicinal wild mushrooms [abstract]. United States-Japan Cooperative Program in Natural Resources. Bio-S#1.
Technical Abstract: We have been trying to develop a bioprocess for the production of polyol oils directly from soybean oil. We reported earlier the polyol products produced from soybean oil by Acinetobacter haemolyticus A01-35 (NRRL B-59985) (Hou and Lin, 2013). The objective of this study is to identify the chemical species of the polyol oil products produced from soybean oil by Pseudomonas aeruginosa E03-12 NRRL B-59991 and compare them with the polyol oils products produced from our previous study Acinetobacter strain. Tricaprylin was selected as internal standard for HPLC quantitative estimation of products. A CombiFlash chromatographic method was established for separation of the polyol oil products. The molecular species of the polyol oils produced from soybean oil by strain E03-12 were identified with HPLC/MS. We identified 41 derivatives of DAG, among them 32 molecular species containing one hydroxy FA and one normal FA, 8 molecular species containing two hydroxy FA without normal FA, and one molecular species containing two normal FA without hydroxylated FA. The hydroxy FA included mono-, di- and tri-hydroxy FA. Eight molecular species of DAG containing one trihydroxy FA and 14 molecular species of DAG containing one dihydroxy FA. We have also identified 64 molecular species of TAG, among them 13 molecular species containing two hydroxy FA, 42 molecular species containing one hydroxy FA and 9 molecular species containing no hydroxylated FA. This is different from our previous findings with Acinetobacter haemolyticus A01-35 which produced only DAG polyol oils.