Production of polyol oils from soybean oil by Pseudomonas aeruginosa E03-12.

Authors: Hou, Ching; Lin, Jiann; Ray, Karen

Submitted to: American Oil Chemists' Society Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 12/31/2015
Publication Date: 5/3/2016
Citation: Hou, C.T., Lin, J.T., Ray, K.J. 2016. Production of polyol oils from soybean oil by Pseudomonas aeruginosa E03-12 [abstract]. American Oil Chemists' Society.

Interpretive Summary:

Technical Abstract: Soy-polyols are important starting materials for the manufacture of polymers such as polyurethane. We have been trying to develop a bioprocess for the production of polyol oils directly from soybean oil. We reported earlier the polyol products produced from soybean oil by Acinetobacter haemolyticus A01-35 (NRRL B-59985). In our continuous screening, we found another culture Pseudomonas aeruginosa E03-12 NRRL B-59991 which produced more polyol oils. We identified the products polyol oils as 41 derivatives of DAG, among them 32 molecular species containing one hydroxy FA and one normal FA, 8 molecular species containing two hydroxy FA without normal FA, and one molecular species containing two normal FA without hydroxylated FA. The hydroxy FA included mono-, di- and tri-hydroxy FA. Eight molecular species of DAG containing one trihydroxy FA and 14 molecular species of DAG containing one dihydroxy FA. We have also identified 64 molecular species of TAG, among them 13 molecular species containing two hydroxy FA, 42 molecular species containing one hydroxy FA and 9 molecular species containing no hydroxylated FA. This is different from our previous findings with Acinetobacter haemolyticus A01-35 which produced only DAG polyol oils.