|CHANG, SHUANG - Shandong Agricultural University|
|XIE, QINGMEI - South China Agricultural University|
|HE, YANGHUA - University Of Maryland|
|DONG, KUNZHE - Orise Fellow|
|ERNST, CATHERINE - Michigan State University|
|SONG, JINZHOU - University Of Maryland|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/9/2015
Publication Date: 1/9/2016
Citation: Zhang, H., Chang, S., Xie, Q., He, Y., Dong, K., Ernst, C.W., Song, J. 2016. Differential expression profiles of miRNAs induced by vaccination followed by Marek’s disease virus challenge at cytolytic stage in chickens resistant or susceptible to Marek’s disease. In Proceedings of: International Conference of Plant and Animal Genome, January 8-13, 2016, San Diego, California. W776.
Technical Abstract: Mounting evidence shows microRNAs (miRNAs) directly regulate gene expression post-transcriptionally through base-pairing with regions in the 3’-untranslated sequences of target gene mRNAs, which results in dysregulation of gene expression/translation and subsequently modulates cellular processes. We reported earlier that Marek’s disease (MD) virus (MDV) induced significantly differentiated expression of 44 and 71 miRNAs in the line 63 MD resistant and line 72 susceptible chickens, respectively. We then reported 58 and 17 differentially expressed miRNAs induced by HVT, 17 and 57 miRNAs induced by CVI988/Rispens in the line 63 and 72 chickens, respectively. This study was designed to identify miRNAs differentially expressed in spleen in response to vaccination followed by MDV challenge at cytolytic stage by small RNA deep sequencing. Twenty four and 36 differentially expressed miRNAs were identified in response to HVT followed by MDV challenge, while Rispens and MDV induced 6 and 31 differentially expressed miRNAs in lines 63 and 72, respectively, in contrast to the control counterparts (Log2 fold change = 2.0). Hundreds up to thousands of target genes were predicted for the differentially expressed miRNAs. Over thirty pathways were likely involved with the lists of target genes of the differentially expressed miRNAs, which included TGF-beta signaling, MARK signaling, and Wnt signaling pathways etc., suggesting that the differentially expressed miRNAs are likely to play important roles in immunity, vaccinal protection, and suppression of tumorigenesis against MD through complicated networks. It is anticipated that further studies in extension of this project should lead to better understanding on how microRNAs mediate vaccine protective efficacy in chickens.