|YAN, FANG - Vanderbilt University Medical Center|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/2/2015
Publication Date: N/A
Technical Abstract: The probiotic bacteria, Lactobacillus rhamnosus GG (LGG), has shown beneficial effects on human health, and is accepted by increasing populations for the prevention and treatment of irritable bowel diseases. To increase the bioavailability and efficacy of LGG, the probiotic was encapsulated in hydrogel beads made from pectin, a food grade polysaccharide, glucose and calcium chloride. The encapsulation increased LGG’s acid stability, protected LGG from protease digestion, and promoted the production of p40 and p75, two known LGG-derived proteins involved in its beneficial effects on intestinal homeostasis. To evaluate the effects of encapsulation on LGG prevention of intestinal injury and colitis, mice were treated with dextran sulphate sodium (DSS) for 4 days with the co-treatment of LGG with and without encapsulation. Administration of encapsulated LGG, but not un-encapsulated LGG prevents DSS-induced colonic injury and colitis. These data suggest that the encapsulation approach developed in this study enhances LGG’s survival and efficacy for protection of the intestine from inflammatory disorders.