Submitted to: High Throughput Screening for Food Safety Assessment, Biosensor Technologies: Hyperspectral Imaging and Practical Applications
Publication Type: Book / Chapter
Publication Acceptance Date: 9/23/2014
Publication Date: 9/25/2014
Citation: Gehring, A.G. 2014. Immunological biosensing of foodborne pathogenic bacteria using electrochemical and light-addressable potentiometric sensor (LAPS) detection platforms. In: Bhunia, M. Kim, & Taitt (eds). High Throughput Screening for Food Safety Assessment, Biosensor Technologies: Hyperspectral Imaging and Practical Applications. 1. 1st Edition. elsevier. p.301-314.
Technical Abstract: The US Centers for Disease Control and Prevention estimates that contaminated foods account for 48 million illnesses, 128,000 hospitalizations, and 3000 deaths per year in the United States alone. Of these cases,9.4 million have been attributed to 31 major foodborne pathogens. Microbial culture-based tests are the “gold standard” for detection and identification of pathogenic bacteria in foods. These methods combine growth enrichment in broth culture with plate culture on selective and/or differential agars, as well as biochemical and molecular methods for confirmatory analysis. Though sensitive enough to detect a single, specific bacterium, they typically require days and sometimes weeks to complete and typically do not produce quantitative data unless the even more labor-intensive most probable number technique is employed. Therefore, faster and simpler detection alternatives are ideally required as tools for rapid screening of foods for the presence of harmful microorganisms such as pathogenic bacteria including Escherichia coli O157:H7 and Salmonella.