Location: Avian Disease and Oncology ResearchTitle: LincRNAs in CD4+ T cells and Marek’s disease) Author
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2014
Publication Date: 7/20/2014
Citation: He, Y., Ding, Y., Zhang, H., Cheng, H.H., Zhao, K., Song, J. 2014. LincRNAs in CD4+ T cells and Marek’s disease [abstract]. In: Proceeding of 10th International Symposium on Marek's Disease and Avian Herpesviruses, July 20-23, 2014, East Lansing, Michigan. p. 46. Interpretive Summary:
Technical Abstract: Marek’s disease (MD) is a T cell lymphoma of domestic chickens induced by Marek’s disease virus (MDV) and causes severe economic loss. The MDV is a naturally oncogenic, highly contagious, and cell associated alpha-herpesvirus. For a long time, resistance to MD and disease risk have long been thought to be influenced both by genetic and environmental factors, the combination of which contributes to the observed outcome in an individual. To efficiently control MD, further exploration for the mechanisms of host-virus interactions is needed. Previously, we showed genetic and epigenetic changes of genome in the host are associated with disease resistance or susceptibility. Currently, we found long intergenic noncoding RNAs (lincRNAs) exhibit association with diseases and play important roles in a wide range of biological processes. Based on the transcriptome sequencing data, we identified candidate lincRNAs in CD4+ T cells isolated from two reciprocal cross strains of two highly inbred lines of chickens and analyzed the differential expression of these lincRNAs between the two reciprocal cross strains. Seventy candidate lincRNA loci in CD4+ T cell were identified and 18 significantly and differentially expressed lincRNAs were found and validated by real-time PCR. Subsequently, 70% of the differentially expressed lincRNAs were confirmed between the two reciprocal cross strains. We will validate several vital lincRNAs and further explore their functions to reveal the mechanism of MD mediated lincRNA regulation. We believe that the exploration of lincRNAs will help us understand how lincRNAs facilitate dynamic changes in epigenetics and transcriptome in relation of resistance to Marek’s disease, which, in turn, should reveal in a deep insight on breeding for disease resistance in chickens.