Location: Avian Disease and Oncology ResearchTitle: Differential expression profiling of miRNAs between Marek’s disease resistant and susceptible chickens Author
|Black Pyrkosz, Alexis|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2014
Publication Date: 7/20/2014
Citation: Zhang, H., Xie, Q., Chang, S., He, Y., Ernst, C., Heidari, M., Black Pyrkosz, A.A., Song, J. 2014. Differential expression profiling of miRNAs between Marek’s disease resistant and susceptible chickens [abstract]. In: Proceeding of 10th International Symposium on Marek's Disease and Avian Herpesviruses, July 20-23, 2014, East Lansing, Michigan. p.47. Interpretive Summary:
Technical Abstract: Mounting evidence indicates microRNAs (miRNAs) play important roles in various biological processes including all aspects of cancer biology. The aim of this study was to profile and to assess the differences of miRNAs between the treatment groups of two lines of White Leghorns with or without viral challenge. One of the lines is known as the line 63, resistant to Marek’s disease (MD), and the other, line 72, highly susceptible to MD. Small RNA libraries were prepared using pooled total RNA samples of each treatment group, and sequenced on an Illumina HiSeq 2000 system. Over 18 and 27 million filtered sequence reads were extracted for the control groups of the lines 63 and 72; and over 24 and 20 million reads for the MD virus (MDV) challenged groups of the lines, respectively. A total of 498 and 502 known and novel miRNAs were identified in the control groups of lines 63 and 72, respectively. The reads ranged from 5 to 951,041 per identified miRNA in line 63 and 5 to 606,219 in line 72. In contrast, 318 and 343 known and novel miRNAs were detected in the MDV challenged groups of lines 63 and 72 with number of expressed reads ranging from 5 to 926,520 and 5 to 716,800, respectively. The expressions of 34 and 21 miRNA genes significantly differed between the two lines in the control and the MDV challenged groups, respectively (Log2 Fold =1.5; P =0.01). In contrast to the control groups, MDV induced 45 and 47 known and novel miRNAs that were differentially expressed in lines 63 and 72, respectively. The findings may lead to better understanding on epigenetic roles played in disease resistance.