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United States Department of Agriculture

Agricultural Research Service


Location: Renewable Product Technology Research

Title: Development of mutated Kluyveromyces marxianus strains for ethanol production at elevated temperature from biomass hydrolysate)

item Hughes, Stephen
item Cox, Elby
item Kurtzman, Cletus
item Bischoff, Kenneth
item Liu, Siqing
item Cote, Gregory - Greg
item Rich, Joseph
item Bang, Sookie
item Pinkleman, Rebecca
item Riano-herrera, Nester
item Rodriguez-valencia, Nelson
item Lopez-nunez, Juan

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/7/2013
Publication Date: 5/7/2013
Citation: Hughes, S.R., Cox, E.J., Kurtzman, C.P., Bischoff, K.M., Liu, S., Cote, G.L., Rich, J.O., Bang, S.S., Pinkleman, R., Riano-Herrera, N.M., Rodriguez-Valencia, N., Lopez-Nunez, J.C. 2013. Development of mutated Kluyveromyces marxianus strains for ethanol production at elevated temperature from biomass hydrolysate [abstract]. Center for Process Analysis and Control.

Interpretive Summary:

Technical Abstract: The yeast K. marxianus has advantages over the most commonly used industrial ethanologen, Saccharomyces cerevisiae, such as the ability to grow at 47°C, to produce ethanol at temperatures above 40°C, and to grow on a wide variety of substrates, including starch, sucrose, pectins, and cellulosic biomass, that make it a promising candidate for development as a versatile, thermotolerant, industrial ethanologen. To improve its growth and ethanol yield at elevated temperature under microaerophilic conditions, wild-type K. marxianus NRRL Y 1109 was irradiated with UV C and the irradiated cultures were selected for growth at elevated temperature using automated protocols on a robotic platform for picking and spreading the cultures and for processing the resulting plates. Two mutant strains were isolated that grew aerobically on glucose at 47°C and anaerobically at 46°C on a wide variety of substrates that are constituents of biomass materials commonly considered feedstocks for renewable fuels. These strains gave higher ethanol yields than wild-type using either glucose (0.51 and 0.43 g ethanol/ g glucose, respectively; P=0.014) or galacturonic acid (0.48 and 0.34 g ethanol/ g galacturonic acid, respectively; P<0.00001) as substrate at 46°C.

Last Modified: 8/24/2016
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