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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #288232

Title: Custom-tailored water-insoluble glucans from sucrose via glucansucrases

item Cote, Gregory
item Skory, Christopher - Chris

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/11/2013
Publication Date: 4/11/2013
Citation: Cote, G.L., Skory, C.D. 2013. Custom-tailored water-insoluble glucans from sucrose via glucansucrases [abstract]. American Chemical Society. Abstract CARB34.

Interpretive Summary:

Technical Abstract: Dextrans and related glucans produced from sucrose by lactic acid bacteria have been studied for many years and are used in numerous commercial applications and products. Most of these glucans are water-soluble, except for a few notable exceptions from cariogenic Streptococcus spp. and a very small number of Leuconostoc mesenteroides strains. The ability to produce water-insoluble glucans in situ may be of value in encapsulation technology and in the production of biocompatible films and fibers. There are several different ways in which these enzymes can be exploited to produce water-insoluble glucans with varying properties. We determined that modifying a single amino acid from a L. mesenteroides glucansucrase significantly altered the proportions of linkage types, with correlating changes in the physical properties of the polysaccharide. Furthermore, we can use specific combinations of enzymes to produce graft copolymers with hybrid properties that can be utilized for the production of functional glucans.