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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research

Title: Production of Truncated Candida antarctica Lipase B Gene Using Automated PCR Gene Assembly Protocol and Expression in Yeast for use in Ethanol and Biodiesel Production.)

Author
item Hughes, Stephen
item Moser, Bryan
item Harmsen, Amanda
item Robinson, Samantha
item Bischoff, Kenneth
item Jones, Marjorie
item Pinkelman, Rebecca
item Bang, Sookie
item Tasaki, Ken
item Doll, Kenneth - Ken
item Qureshi, Nasib
item Liu, Siqing
item Saha, Badal
item Jackson Jr, John
item Cotta, Michael
item Rich, Joseph
item Caimi, Paolo

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/28/2010
Publication Date: 4/28/2010
Citation: Hughes, S.R., Moser, B.R., Harmsen, A.J., Robinson, S., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.B., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of truncated Candida antarctica Lipase B gene using automated PCR gene assembly protocol and expression in yeast for use in ethanol and biodiesel production [abstract]. Advanced Biofuels Leadership Conference. Talk 3. p. 5.

Interpretive Summary:

Technical Abstract: An improved column-based process for production of biodiesel was developed using a column containing a strongly basic anion-exchange resin in sequence with a column containing a resin to which a lipase biocatalyst is bound. Currently most biodiesel is produced by transesterification of triglycerides, such as corn oil, with short-chain alcohols, such as methanol or ethanol, with a strong base as a catalyst. The transesterification reaction to produce biodiesel can also be accomplished using resin-bound lipases as biocatalysts. In this study, two columns were prepared, one containing the strongly basic anion-exchange resin DIAION® and the other containing Candida antartica lipase B enzyme bound to Sepabead® EC-EP resin. These columns individually both catalyzed the transesterification of a mixture of ethanol and corn oil, producing comparable yields of ethyl esters (biodiesel). When run in sequence, it was demonstrated that the yield of ethyl esters increased by 20%.

Last Modified: 8/24/2016
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