Location: Hard Winter Wheat Genetics Research
Project Number: 3020-21000-012-003-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2019
End Date: Aug 31, 2024
Objective:
1. Develop algorithms using unsupervised classification methods for fast evaluation of the Fusarium-Damaged Kernels on multiple platforms.
2. Identify resistance genes in Lyman and CI 13227 and DNA markers linked to these genes for marker-assisted selection.
3. Develop hard winter wheat germplasm with a high level of resistance by pyramiding resistance gene Fhb1 with a gene on chromosome 5A and one or two other minor genes into U.S. hard winter wheat using marker-assisted selection and evaluate their individual and combined effects.
Approach:
1. Inoculate a wheat mapping population with FHB and harvest infected plants to get both Fusarium-damaged and healthy kernels, obtain high resolution pictures of harvested mixed kernels from each line, analyze pictures to separate healthy and scabby kernels using a newly developed algorithm in a high-throughput scripting language.
2. Genotype two mapping populations using SNP markers derived from genotyping-by-sequencing; phenotype population for FHB resistance in both greenhouse and field; and conduct linkage mapping and QTL analysis.
3. Make crosses among lines with minor and major genes for FHB tolerance and low DON accumulation, select target genotypes using QTL-linked markers for backcrosses; evaluate selected progeny for FHB resistance in field and greenhouse.
