Location: Hard Winter Wheat Genetics Research
Project Number: 3020-21000-011-040-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2021
End Date: Jul 31, 2024
1. Develop and validate Practical Haplotype Graph (PHG) database for imputation of genotypes in breeding lines. 2. Phenotype FHB-related traits on diverse wheat lines to develop optimized genomic selection (GS) models for FHB resistance. 3. Perform genome-wide association studies (GWAS) to identify functionally resistant haplotypes/quantitative trait loci (QTLs) for FHB resistance. 4. Develop resistance haplotypes into genetic marker assays for marker-assisted selection (MAS) in breeding programs.
Wheat exome capture of FHB-resistant, elite, and breeding lines will be conducted, and genic region variants will be used to populate the PHG database. The database will be used to impute genotypes of lower coverage sequenced lines. Genome-wide variants from all FHB phenotyped lines will be used to optimize a GS model of FHB resistance, and tested on a subset of the adapted lines, and breeding lines from across the Great Plains region. Using the phenotypes of the wheat lines, a GWAS will be conducted to identify associated regions of the genome with FHB resistance. By grouping resistant and susceptible germplasm, we will identify resistant haplotypes to develop more targets for marker-assisted selection.