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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Research Project #438969

Research Project: Develop Functional Markers for Wheat Biotic and Abiotic Stress

Location: Hard Winter Wheat Genetics Research

Project Number: 3020-21000-011-032-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2020
End Date: Aug 31, 2023

Objective:
1. Develop functional markers for Cmc4 gene for wheat curl mite resistance using high resolution mapping. 2. Develop functional markers for Hessian fly resistance genes using genome-wide association analysis

Approach:
1. Use next-generation-sequencing (NGS)-based SNP markers to identify high-density markers in Cmc4 gene region for curl mite resistance, isolate heterozygous inbred families from the current mapping population of OK05312 x SD06165 using flanking markers, convert NGS-SNPS into KASP-based markers for screening of progeny from the heterozygous inbred families, advance segregating progeny from heterozygous inbred family and screen recombinants within the gene region using flanking markers, phenotyping selected recombinants to narrow down the target gene region, and develop markers that are closely linked to the gene for breeding applications. 2. Use high-density next-generation-sequencing-based markers to genotype a panel of 1000 wheat accessions collected worldwide and perform genome-wide association studies. Screen the population for Hessian fly resistance in repeated experiments, perform statistical analysis of genotypic and phenotypic data to identify the QTL regions in the wheat chromosomes associated with Hessian fly resistance, develop markers for these QTLs for marker-assisted selection.