Objective 1: To mitigate issues with bacterial pathogen-contaminated irrigation waters, examine the use of biochar as an antimicrobial and filtration intervention, for example, combining biochar filtration with ARS pre-existing zero-valent water filtration technology. Objective 2: Examine the use of adding biochar to compost piles, in order to inactivate pathogens in the compost, but especially the problematic “toes” of manure piles.
The approach of project will follow two objectives. The first objective will evaluate the ability of biochar filters to remove pathogenic bacteria from surface irrigation waters with or without zero valent iron and sand-composite filtration. Biochar pyrolysis will be optimized for this purpose by altering the residence time, temperature and biofeedstock with an in-house biochar slow-pyrolysis reactor. The optimized water filtration units will then be scaled up to reduce pathogens in irrigation water, lowering the risk of foodborne illness from irrigated fresh produce. Second, pyrolysis will be further optimized for maximal antimicrobial efficacy of biochar. This biochar will then be utilized in lab-scale and field-trial dairy and poultry compost experiments with the goal of more rapidly inactivating EHEC and Salmonella. Successful results will allow for shorter composting times prior to field application, which will decrease the chances for pathogenic bacteria to survive the process and contaminate field crops. Stakeholders will be consulted and collaborated with for all objectives, and technology will be transferred to the appropriate entities. Overall, the results and outcomes from this project plan will increase the safety of fresh fruits and vegetables and lower the burden of human-related illnesses caused by foodborne pathogens by providing practical intervention solutions for farmers, packers, processors and distributers of fresh produce, related to foodborne pathogens.
This is a newly approved NP108 project. Please see 8072-41420-020-00D for annual report. A thorough literature review has been completed, and experiments have been planned.
Richards, G.P., Watson, M.A., Madison, D., Soffer, N., Needleman, D.S., Soroka, D.S., Uknalis, J., Baranzoni, G., Church, K.M., Poison, S.W., Elston, R., Langdon, C., Sulakvelictze, A. 2021. Bacteriophages against Vibrio coralliilyticus and Vibrio tubiashii: Isolation, characterization and remediation of larval oyster mortalities. Applied and Environmental Microbiology. 87. https://doi.org/10.1128/AEM.00008-21.