Location: Hard Winter Wheat Genetics Research
Project Number: 3020-21000-011-020-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2018
End Date: Aug 31, 2022
1. Develop a BSMV-mediated CRISPR/Cas9 genome editing system that bypasses the requirement for transformation and regeneration of tissue culture procedures in wheat. 2. Generate FHB-resistant germplasm by genome modification of a putative susceptibility gene, TaHRC. 3. Develop haplotype markers for important agronomic traits in wheat.
1. Conduct marker-assisted transfer of Cas9 (Cas9-OE) from Bobwhite into locally adapted cultivars by conventional crossing methods. 2. Use Agrobacterium-based BSMV-mediated genome editing system to edit the target gene TaHRC in selected Cas9-expressing plants. 3. Conduct genotypic evaluation of edited plants for target sequence variation and evaluate phenotypes of plants to confirm the function of the edited gene mutants. 4. Convert genotyping-by-sequencing (GBS) or simple sequence repeat (SSR) markers linked to important traits into high-throughput breeder-friendly KASP markers.