Research Project: Development of Detection and Intervention Technologies for Bacterial and Viral Pathogens Affecting Shellfish

Location: Food Safety and Intervention Technologies Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Bacteriophages improve survival and metamorphosis of larval Pacific oysters (Crassostrea gigas) exposed to Vibrio coralliilyticus (strain RE98) - (Peer Reviewed Journal)
Madison, D., Richards, G.P., Sulakvelidze, A., Langdon, C. 2022. Bacteriophages improve survival and metamorphosis of larval Pacific oysters (Crassostrea gigas) exposed to Vibrio coralliilyticus (strain RE98). Aquaculture. 555(2022)738242. https://doi.org/10.1016/j.aquaculture.2022.738242.

Vibrio coralliilyticus induction of virulence toward larval oysters and corals at elevated seawater temperatures and potential mitigation strategies - (Proceedings)
Richards, G.P., Ushijima, B. 2021. Vibrio coralliilyticus induction of virulence toward larval oysters and corals at elevated seawater temperatures and potential mitigation strategies. Proceedings of US-Japan Natural Resources Panel on Aquaculture. p. 104-110.

Evaluation of SDS and GRAS liquid disinfectants for mitigation of potential HAV contamination of berries - (Peer Reviewed Journal)
Kingsley, D.H., Annous, B.A. 2021. Evaluation of SDS and GRAS liquid disinfectants for mitigation of potential HAV contamination of berries. Journal of Applied Microbiology. 15123. https://doi.org/10.1111/jam.15123.

Bacteriophages against Vibrio coralliilyticus and Vibrio tubiashii: Isolation, characterization and remediation of larval oyster mortalities - (Peer Reviewed Journal)
Richards, G.P., Watson, M.A., Madison, D., Soffer, N., Needleman, D.S., Soroka, D.S., Uknalis, J., Baranzoni, G., Church, K.M., Poison, S.W., Elston, R., Langdon, C., Sulakvelictze, A. 2021. Bacteriophages against Vibrio coralliilyticus and Vibrio tubiashii: Isolation, characterization and remediation of larval oyster mortalities. Applied and Environmental Microbiology. 87. https://doi.org/10.1128/AEM.00008-21.

Efficacy of chlorine dioxide gas against hepatitis A virus on blueberries, blackberries, raspberries, and strawberries - (Peer Reviewed Journal)
Annous, B.A., Buckley, D., Kingsley, D.H. 2021. Efficacy of chlorine dioxide gas against hepatitis A virus on blueberries, blackberries, raspberries, and strawberries. Food and Environmental Virology. 13,241-247. https://doi.org/10.1007/s12560-021-09465-1.

Seasonal and geographical differences in total and pathogenic vibrio parahaemolyticus and vibrio vulninficus levels in seawater and oysters from the Delaware and Chesapeake Bays using several methods - (Peer Reviewed Journal)
Parveen, S., Jacobs, J., Ozbay, G., Chintapenta, L., Almuhaideb, E., Meredith, J., Ossai, S., Abbott, A., Grant, A., Brohawn, K., Chigbu, P., Richards, G.P. 2020. Seasonal and geographical differences in total and pathogenic vibrio parahaemolyticus and vibrio vulninficus levels in seawater and oysters from the Delaware and Chesapeake Bays using several methods. Applied and Environmental Microbiology. 86(23). https://doi.org/10.1128/AEM.01581-20.

Detection of Hepatitis A virus and other Enteric viruses in shellfish collected in the Gulf of Naples, Italy - (Peer Reviewed Journal)
Fusco, G., Boccia, F., Kingsley, D.H., Amoroso, M.G., Cioffi, B., Rossi, R., Fratamico, P., Larosa, G., Anastasio, A., Pepe, T. 2019. Detection of Hepatitis A virus and other Enteric viruses in shellfish collected in the Gulf of Naples, Italy. International Journal of Environmental Research and Public Health. 16:2588.

Development and evaluation of polymorphic locus sequence typing for epidemiological tracking of Vibrio parahaemolyticus - (Peer Reviewed Journal)
Edlind, T., Richards, G.P. 2019. Development and evaluation of polymorphic locus sequence typing for epidemiological tracking of Vibrio parahaemolyticus. Foodborne Pathogens and Disease. https://doi.org/10.1089/fpd.2019.2649.

Evaluation of a male-specific DNA coliphage persistence within Eastern oysters (Crassostrea virginica) - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H., Annous, B.A., Meade, G.K. 2019. Evaluation of a male-specific DNA coliphage persistence within Eastern oysters (Crassostrea virginica). Food and Environmental Virology. 11:120-125.

Bacteriophages against pathogenic vibrios in Delaware Bay oysters (Crassostrea virginica) during a period of high levels of pathogenic vibrio parahaemolyticus - (Peer Reviewed Journal)
Richards, G.P., Chintapenta, L.K., Watson, M.A., Abbott, A.G., Ozbay, G., Uknalis, J., Oyelade, A., Parveen, S. 2019. Bacteriophages against pathogenic vibrios in Delaware Bay oysters (Crassostrea virginica) during a period of high levels of pathogenic vibrio parahaemolyticus. Food and Environmental Virology. https://doi.org/10.1007/s12560-019-09365-5.

Whole genome sequences of two Pseudoalteromonas piscicida strains DE1-A and DE2-A, which exhibit strong antibacterial activity against Vibrio vulnificus - (Peer Reviewed Journal)
Richards, G.P., Needleman, D.S., Watson, M.A., Poulson, S.W. 2019. Whole genome sequences of two Pseudoalteromonas piscicida strains DE1-A and DE2-A, which exhibit strong antibacterial activity against Vibrio vulnificus. Microbiology Resource Announcements. 8:e01451-18.

Complete genome sequence of Vibrio coralliilyticus RE22, a marine bacterium pathogenic toward larval shellfish - (Peer Reviewed Journal)
Richards, G.P., Kingham, B., Shevchenko, O., Watson, M.A., Needleman, D.S. 2018. Complete genome sequence of Vibrio coralliilyticus RE22, a marine bacterium pathogenic toward larval shellfish. Microbiology Resource Announcements. 7(17):e01332-18. https://doi.org/10.1128/MRA01332-18.

Oxygen-dependent laser inactivation of murine norovirus using visible light lasers - (Peer Reviewed Journal)
Kingsley, D.H., Johnson, A., Robinson, K., Perez, R., Basaldua, I., Burkins, P., Marcano, A. 2018. Oxygen-dependent laser inactivation of murine norovirus using visible light lasers. Virology Journal. 15:117. https://doi.org/10.1186/s12985-018-1019-2.

Factors affecting infection of corals and larval oysters by vibrio coralliilyticus - (Peer Reviewed Journal)
Ushijima, B., Richards, G.P., Watson, M.A., Schubiger, C.B., Hase, C.C. 2018. Factors affecting infection of corals and larval oysters by vibrio coralliilyticus. PLoS One. https://doi.org/10.1371/journal.pone.0199475.

Inactivation of tulane virus on blueberries with gaseous chlorine dioxide - (Abstract Only)

Evaluation of gaseous chlorine dioxide for the inactivation of tulane virus on blueberries - (Peer Reviewed Journal)
Kingsley, D.H., Perez, R., Niemira, B.A., Fan, X. 2018. Evaluation of gaseous chlorine dioxide for the inactivation of tulane virus on blueberries. International Journal of Food Microbiology. 273:23-32.

Evaluation of 405 nm monochromatic light for inactivation of tulane virus on blueberry surfaces - (Peer Reviewed Journal)
Kingsley, D.H., Perez, R.E., Boyd, G., Sites, J.E., Niemira, B.A. 2018. Evaluation of 405 nm monochromatic light for inactivation of tulane virus on blueberry surfaces. Journal of Applied Microbiology. 124:1017-1022.

Three draft genomes of Vibrio coralliilyticus strains isolated from bivalve hatcheries - (Peer Reviewed Journal)
Kehlet-Delgado, H., Richards, G.P., Hase, C., Mueller, R.S. 2017. Three draft genomes of Vibrio coralliilyticus strains isolated from bivalve hatcheries. Genome Announcements. Volume 5: Issue 41 e01162-17.

Evaluation of chlorine treatment levels on inactivation of human norovirus and MS2 bacteriophage during sewage treatment - (Peer Reviewed Journal)
Kingsley, D.H., Fay, J., Calci, K., Pouillot, R., Woods, J., Chen, H., Niemira, B.A., Van Doren, J.M. 2017. Evaluation of chlorine treatment levels on inactivation of human norovirus and MS2 bacteriophage during sewage treatment. Applied and Environmental Microbiology. 83:e01270-17.

Persistence of MS-2 bacteriophage within Eastern Oysters - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H., Meade, G.K. 2017. Persistence of MS-2 bacteriophage within Eastern Oysters. Food and Environmental Virology. 10:83-88.

Complete genome sequence of Pseudoalteromononas piscicida strain DE2-B, a bacterium with broad inhibitory activity toward human and fish pathogens - (Peer Reviewed Journal)
Richards, G.P., Needleman, D.S., Watson, M.A. 2017. Complete genome sequence of Pseudoalteromononas piscicida strain DE2-B, a bacterium with broad inhibitory activity toward human and fish pathogens. Genome Announcements. http://doi.org/10.1128/genomeA.00752-17.

Pseudomonas piscicida kills vibrios by two distinct mechanisms - (Abstract Only)
Richards, G.P. 2017. Pseudomonas piscicida kills vibrios by two distinct mechanisms. Meeting Abstract. doi.org/10.1128/AEM00800-17.

High pressure processing's potential to inactivate norovirus and other fooodborne viruses - (Book / Chapter)
Kingsley, D.H. 2017. High pressure processing's potential to inactivate norovirus and other fooodborne viruses. In: Nova Science Publishers. Noroviruses: Outbreaks Contral and Prevention Strategies. Jesus Romalde. p. 173-221.

Evaluation of 405nm CW visible blue light as a means of inactivating Tulane Virus on Blueberries - (Abstract Only)
Kingsley, D.H., Perez-Perez, R.E., Boyd, G., Sites, J.E., Niemira, B.A. 2017. Evaluation of 405nm CW visible blue light as a means of inactivating Tulane Virus on Blueberries.[Abstract]. International Association for Food Protection., Tampa, Florida., Volume 1, Page 1.

Mechanisms for pseudoalteromonas piscicida-induced killing of vibrios and other bacterial pathogens - (Peer Reviewed Journal)
Richards, G.P., Watson, M.A., Needleman, D.S., Uknalis, J., Boyd, E., Fay, J.P. 2017. Mechanisms for pseudoalteromonas piscicida-induced killing of vibrios and other bacterial pathogens. Applied and Environmental Microbiology. doi: 10.1128/AEM.00175-17.

Development of one-step Loop-Mediated Isothermal Amplification (LAMP) for the detection of norovirus in oysters - (Peer Reviewed Journal)
Jeon, S., Seo, D., Oh, H., Kingsley, D.H., Choi, C. 2017. Development of one-step Loop-Mediated Isothermal Amplification (LAMP) for the detection of norovirus in oysters. Food Control. 73:1002-1009.