Research Project: Advance the Development of Technologies for Detecting and Determining the Stability and Bioavailability of Toxins that Impact Food Safety and Food Defense

Location: Foodborne Toxin Detection and Prevention Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Ex vivo and in vitro methods for detection of bioactive staphylococcal enterotoxins - (Book / Chapter)
Rasooly, R., Do, P.M., Hernlem, B.J. 2022. Ex vivo and in vitro methods for detection of bioactive staphylococcal enterotoxins. In: Ossandon, M.R., Baker, H., Rasooly, A., editors. Biomedical Engineering Technologies. Volume 1. New York, NY: Humana Press. p. 237-255. https://doi.org/10.1007/978-1-0716-1803-5_13.

In vivo activity of repurposed amodiaquine as a host-targeting therapy for the treatment of anthrax - (Peer Reviewed Journal)
Shilman, M.M., Bartolo, G., Alameh, S., Slay, R., Peterson, J., Lawrence, W., Peel, J., Sivasubramani, S., Beasley, D., Cote, C., Demons, S., Halasahoris, S., Miller, L., Klimko, C., Shoe, J., Fetterer, D., Mccomb, R., Ho, C., Bradley, K., Hartmann, S., Cheng, L.W., Chugunova, M., Kao, C., Tran, J., Derbedrossian, A., Zilbermintz, L., Amali-Adekwu, E., Levitin, A., West, J. 2021. In vivo activity of repurposed amodiaquine as a host-targeting therapy for the treatment of anthrax. ACS Infectious Diseases. 7(8):2176-2191. https://doi.org/10.1021/acsinfecdis.1c00190.

Validation of a cell-based assay for detection of active Shiga toxins produced by Escherichia coli in water - (Peer Reviewed Journal)
Hughes, A.C., Patfield, S., Rasooly, R., He, X. 2020. Validation of a cell-based assay for detection of active Shiga toxins produced by Escherichia coli in water. International Journal of Environmental Research and Public Health. 17(21):7901. https://doi.org/10.3390/ijerph17217901.

Quantitative bioluminescence assay for measuring Bacillus cereus nonhemolytic enterotoxin complex - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2020. Quantitative bioluminescence assay for measuring Bacillus cereus nonhemolytic enterotoxin complex. PLoS ONE. 15(9): Article e0238153. https://doi.org/10.1371/journal.pone.0238153.

Low prevalence of mobile colistin-resistance in U.S. meat, catfish, poultry and genomic characterization of a mcr-1 positive Escherichia coli strain - (Peer Reviewed Journal)
Wang, Y., Hou, N., Johnston, J., Sarreal, C., Jarosh, J., Hughes, A.C., Gu, Y.Q., He, X. 2020. Low prevalence of mobile colistin-resistance in U.S. meat, catfish, poultry and genomic characterization of a mcr-1 positive Escherichia coli strain. Food Control. 118. https://doi.org/10.1016/j.foodcont.2020.107434.

whISOBAXTM inhibits bacterial pathogenesis and enhances the effect of antibiotics - (Peer Reviewed Journal)
Rasooly, R., Choi, H., Do, P.M., Morroni, G., Brescini, L., Cironi, O., Giacometti, A., Apostolidis, E. 2020. whISOBAXTM inhibits bacterial pathogenesis and enhances the effect of antibiotics. Antibiotics. 9(5):264. https://doi.org/10.3390/antibiotics9050264.

Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms - (Peer Reviewed Journal)
Bever, C.R., Adams, C.A., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2020. Lateral flow immunoassay (LFIA) for the detection of lethal amatoxins from mushrooms. PLoS One. 15(4). https://doi.org/10.1371/journal.pone.0231781.

Investigation of the immunogenicity of Zika glycan loop - (Peer Reviewed Journal)
Henderson, E., Tam, C.C., Cheng, L.W., Ngono, A.E., Nguyen, A., Shresta, S., McGee, M., Padgett, H., Gill, L., Martchenko, M. 2020. Investigation of the immunogenicity of Zika glycan loop. Virology Journal. 17:43. https://doi.org/10.1186/s12985-020-01313-1.

CCD based detector for detection of Abrin toxin activity - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2020. CCD based detector for detection of Abrin toxin activity. Toxins. 12(2). https://doi.org/10.3390/toxins12020120.

Rapid, sensitive, and accurate point-of-care detection of lethal amatoxins in urine - (Peer Reviewed Journal)
Bever, C.R., Swanson, K.D., Hamelin, E.I., Filigenzi, M., Poppenga, R., Kaae, J., Cheng, L.W., Stanker, L.H. 2020. Rapid, sensitive, and accurate point-of-care detection of lethal amatoxins in urine. Toxins. 12(2):123. https://doi.org/10.3390/toxins12020123.

Witch hazel significantly improves the efficacy of commercially available teat dips - (Peer Reviewed Journal)
Rasooly, R., Molnar, A., Do, P.M., Marroni, G., Brescini, L., Cirioni, O., Giavometti, A., Apostolidis, E. 2020. Witch hazel significantly improves the efficacy of commercially available teat dips. Pathogens. 9(2). Article 92. https://doi.org/10.3390/pathogens9020092.

Structural and functional characterization of Stx2k, a new subtype of Shiga toxin 2 - (Peer Reviewed Journal)
Hughes, A.C., Zhang, Y., Bai, X., Xiong, Y., Wang, Y., Yang, X., Xu, Q., He, X. 2019. Structural and functional characterization of Stx2k, a new subtype of Shiga toxin 2. Microorganisms. 8(1):4. https://doi.org/10.3390/microorganisms8010004.

The novel Clostridial neurotoxin produced by strain IBCA10-7060 is immunologically equivalent to BoNT/HA - (Peer Reviewed Journal)
Fan, Y., Barash, J.R., Conrad, F., Lou, J., Tam, C.C., Cheng, L.W., Arnon, S.S., Marks, J.D. 2019. The novel Clostridial neurotoxin produced by strain IBCA10-7060 is immunologically equivalent to BoNT/HA. Toxins. 12(1):9. https://doi.org/10.3390/toxins12010009.

A rapid extraction method combined with a monoclonal antibody-based immunoassay for the detection of amatoxins - (Peer Reviewed Journal)
Bever, C.R., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2019. A rapid extraction method combined with a monoclonal antibody-based immunoassay for the detection of amatoxins. Toxins. 11(12). https://doi.org/10.3390/toxins11120724.

In-vitro inhibition of staphylococcal pathogenesis by witch-hazel and green tea extracts - (Peer Reviewed Journal)
Rasooly, R., Molnar, A., Choi, H., Do, P.M., Racicot, K., Apostolidis, E. 2019. In-vitro inhibition of staphylococcal pathogenesis by witch-hazel and green tea extracts. Antibiotics. 8(4):244. https://doi.org/10.3390/antibiotics8040244.

Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China - (Peer Reviewed Journal)
Yang, X., Bai, X., Zhang, J., Sun, H., Fu, S., Fan, R., He, X., Scheutz, F., Matussek, A., Xiong, Y. 2020. Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China. International Journal of Medical Microbiology. 310(1):151377. https://doi.org/10.1016/j.ijmm.2019.151377.

Particulate Shiga Toxin 2 in blood is associated to the development of hemolytic uremic syndrome in children - (Peer Reviewed Journal)
Brigotti, M., He, X., Carnicelli, D., Arfilli, V., Porcellini, E., Galassi, E., Tazzari, P., Ricci, F., Patfield, S.A., Testa, S., Paglialonga, F., Picicco, D., Caprioli, A., Scavia, G., Morabito, S., Ardissino, G. 2019. Particulate Shiga Toxin 2 in blood is associated to the development of hemolytic uremic syndrome in children. Thrombosis and Haemostasis. 120(1):107-120. https://doi.org/10.1055/s-0039-3400301.

A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure - (Peer Reviewed Journal)
Riangrungroj, P., Bever, C.R., Hammock, B.D., Polizzi, K.M. 2019. A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure. Scientific Reports. 9:12466. https://doi.org/10.1038/s41598-019-48907-6.

Prebiotics, probiotics, and bacterial infections - (Book / Chapter)
Tam, C.C., Land, K.M., Cheng, L.W. 2019. Prebiotics, probiotics, and bacterial infections. In: Franco-Robles, E. and Ramírez-Emiliano, J., editors. Prebiotics and Probiotics, Potential Benefits in Nutrition and Health. IntechOpen. https://doi.org/10.5772/intechopen.89052.

Development and characterization of monoclonal antibodies to botulinum neurotoxin type E - (Peer Reviewed Journal)
Bever, C.R., Scotcher, M., Cheng, L.W., Hnasko, R.M., Stanker, L. 2019. Development and characterization of monoclonal antibodies to botulinum neurotoxin type E. Toxins. 11:407. https://doi.org/10.3390/toxins11070407.

T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2019. T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals. Toxins. 11:399. https://doi.org/10.3390/toxins11070399.

Comparison of soybean peroxidase with horseradish peroxidase and alkaline phosphatase used in immunoassays - (Peer Reviewed Journal)
Yang, H., Bever, C.R., Zhang, H., Mari, G.M., Li, H., Zhang, X., Guo, L., Wang, Z., Luo, P., Wang, Z. 2019. Comparison of soybean peroxidase with horseradish peroxidase and alkaline phosphatase used in immunoassays. Analytical Biochemistry. 581:113336. https://doi.org/10.1016/j.ab.2019.06.007.

Influence of food matrices on the stability and bioavailability of abrin - (Peer Reviewed Journal)
Tam, C.C., Henderson II, T.D., Stanker, L.H., Cheng, L.W. 2018. Influence of food matrices on the stability and bioavailability of abrin. Toxins. 10(12):52. https://doi.org/10.3390/toxins10120502.

Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2018. Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A. Toxins. 10(12):540. https://doi.org/10.3390/toxins10120540.

A rapid, sensitive, and portable biosensor assay for the detection of botulinum neurotoxin serotype A in complex food matrices - (Peer Reviewed Journal)
Tam, C.C., Flannery, A.R., Cheng, L.W. 2018. A rapid, sensitive, and portable biosensor assay for the detection of botulinum neurotoxin serotype A in complex food matrices. Toxins. 10(11):476. https://doi.org/doi:10.3390/toxins10110476.

Synthesis of pyrrolidine-based hamamelitannin analogues as quorum sensing inhibitors in Staphylococcus aureus - (Peer Reviewed Journal)
Rasooly, R., Bouton, J., Van Hecke, K., Van Calenbergh, S. 2018. Synthesis of pyrrolidine-based hamamelitannin analogues as quorum sensing inhibitors in Staphylococcus aureus. Beilstein Journal of Organic Chemistry. 14:2822-2828. https://doi.org/10.3762/bjoc.14.260.

Soluble toll-like receptor 4 impairs the interaction of Shiga toxin 2a with human serum amyloid P component - (Peer Reviewed Journal)
Brigotti, M., Arfilli, V., Carnicelli, D., Ricci, F., Tazzari, P., Ardissino, G., Scavia, G., Morabito, S., He, X. 2018. Soluble toll-like receptor 4 impairs the interaction of Shiga toxin 2a with human serum amyloid P component. Toxins. 10(9):379. https://doi.org/10.3390/toxins10090379.

A new conjugation method used for the development of an immunoassay for amanitin, a deadly mushroom toxin - (Peer Reviewed Journal)
Bever, C.R., Barnych, B., Hnasko, R.M., Cheng, L.W., Stanker, L.H. 2018. A new conjugation method used for the development of an immunoassay for amanitin, a deadly mushroom toxin. Toxins. 10(7):265. https://doi.org/10.3390/toxins10070265.

Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection - (Peer Reviewed Journal)
Rasooly, R., Magoz, Z., Luo, J., Do, P.M., Hernlem, B.J. 2018. Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection. Desalination and Water Treatment. 118:120-125. https://doi.org/10.5004/dwt.2018.22667.

Botulinum neurotoxin detection methods for public health response and surveillance - (Review Article)
Thirunavukkarasu, N., Johnson, E., Pillai, S., Hodge, D., Stanker, L.H., Wentz, T., Singh, B., Venkateswaran, V., Mcnutt, P., Adler, M., Brown, E., Hammack, T., Burr, D., Sharma, S. 2018. Botulinum neurotoxin detection methods for public health response and surveillance. Frontiers in Bioengineering and Biotechnology. 6:80. https://doi.org/10.3389/fbioe.2018.00080.

Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype - (Peer Reviewed Journal)
Bai, X., Fu, S., Zhang, J., Fan, R., Xu, Y., Sun, H., He, X., Xu, J., Xiong, Y. 2018. Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype. Scientific Reports. 8:6756. https://doi.org/10.1038/s41598-018-25233-x.

A single tri-epitopic antibody virtually recapitulates the potency of a combination of three monoclonal antibodies in neutralization of Botulinum Neurotoxin Serotype A - (Peer Reviewed Journal)
Lou, J., Wen, W., Conrad, F., Meng, Q., Dong, J., Sun, Z., Garcia-Rodriguez, C., Farr-Jones, S., Cheng, L.W., Henderson Ii, T.D., Brown, J., Smith, T.J., Smith, L.A., Cormier, A., Marks, J.D. 2018. A single tri-epitopic antibody virtually recapitulates the potency of a combination of three monoclonal antibodies in neutralization of Botulinum Neurotoxin Serotype A. Toxins. 10(2):84. doi: 10.3390/toxins10020084.

An improved method for the sensitive detection of Shiga toxin 2 in human serum - (Peer Reviewed Journal)
He, X., Ardissino, G., Patfield, S.A., Cheng, L.W., Silva, C.J., Brigotti, M. 2018. An improved method for the sensitive detection of Shiga toxin 2 in human serum. Toxins. 10(2):59. https://doi.org/10.3390/toxins10020059.

Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products - (Peer Reviewed Journal)
Hu, L., Ma, L., Zheng, S., He, X., Hammack, T.S., Brown, E.W., Zhang, G. 2018. Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products. Food Control. 88:190-197. https://doi.org/10.1016/j.foodcont.2018.01.006.

Detection of abrin holotoxin using novel monoclonal antibodies - (Peer Reviewed Journal)
He, X., Patfield, S.A., Cheng, L.W., Stanker, L.H., Rasooly, R., McKeon, T.A., Zhang, Y., Brandon, D.L. 2017. Detection of abrin holotoxin using novel monoclonal antibodies. Toxins. 9(12):386. https://doi.org/10.3390/toxins9120386.

TCR-Vß8 as alternative to animal testing for quantifying active SEE - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2017. TCR-Vß8 as alternative to animal testing for quantifying active SEE. Journal of Environmental and Analytical Toxicology. 7(6):527. https://doi.org/10.4172/2161-0525.1000527.

Screening for the presence of mcr-1/mcr-2 genes in Shiga toxin-producing Escherichia coli recovered from a major produce-production region in California - (Peer Reviewed Journal)
Mavrici, D., Yambao, J.C., Lee, B.G., Quinones, B., He, X. 2017. Screening for the presence of mcr-1/mcr-2 genes in Shiga toxin-producing Escherichia coli recovered from a major produce-production region in California. PLoS One. 12(11):e0187827. https://doi.org/10.1371/journal.pone.0187827.

A monoclonal-monoclonal antibody based capture elisa for abrin - (Peer Reviewed Journal)
Tam, C.C., Cheng, L.W., He, X., Merrill, P.A., Hodge, D., Stanker, L.H. 2017. A monoclonal-monoclonal antibody based capture elisa for abrin. Toxins. 9(10):328. https://doi.org/10.3390/toxins9100328.

Abrin toxicity and bioavailability after temperature and pH treatment - (Peer Reviewed Journal)
Tam, C.C., Henderson, T.D., Stanker, L.H., He, X., Cheng, L.W. 2017. Abrin toxicity and bioavailability after temperature and pH treatment. Toxins. 9(10):320. https://doi.org/10.3390/toxins9100320.

Butterfly extracts show antibacterial activity - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Balaban, N., Wolferstan, P., Nash, R., Yael, G. 2017. Butterfly extracts show antibacterial activity. Journal of Agricultural and Food Chemistry. 7:467-479. doi:org/10.4236/aim.2017.76036.

Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays - (Peer Reviewed Journal)
He, X., Patfield, S.A., Rasooly, R., Mavrici, D. 2017. Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays. Journal of Immunological Methods. 44:52-56.

Historical perspectives and guidelines for botulinum neurotoxin subtype nomenclature - (Review Article)
Peck, M.W., Smith, T.J., Anniballi, F., Arnon, S.S., Austin, J.W., Bano, L., Bradshaw, M., Cuervo, M.P., Cheng, L.W., Derman, Y., Dorner, B., Dover, N., Fischer, A., Hill, K.K., Kalb, S., Korkeala, H., Lindstrom, M., Lista, F., Luquez, C., Mazuet, C., Pellett, S., Pirazzini, M., Popoff, M.R., Rasetti-Escargueil, C., Rossetto, O., Rummel, A., Sesardic, T., Singh, B., Stringer, S.C. 2017. Historical perspectives and guidelines for botulinum neurotoxin subtype nomenclature. Toxins. 9(1):38. doi: 10.3390/toxins9010038.

Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D. Journal of Food Science. doi: 10.1111/1750-3841.13634.

Host cell interactions of outer membrane vesicle-associated virulence factors of Enterohemorrhagic Escherichia coli O157: intracellular delivery, trafficking and mechanisms of cell injury - (Peer Reviewed Journal)
Bielaszewska, M., Ruter, C., Bauwens, A., Greune, L., Zhang, W., He, X., Lloubes, R., Fruth, A., Kim, K.S., Schmidt, M., Dobrindt, U., Mellmann, A., Karch, H. 2017. Host cell interactions of outer membrane vesicle-associated virulence factors of Enterohemorrhagic Escherichia coli O157: intracellular delivery, trafficking and mechanisms of cell injury. PLoS Pathogens. 13(2):e1006159. doi: 10.1371/journal. ppat. 1006159.

Probiotic microorganisms inhibit epithelial cell internalization of botulinum neurotoxin serotype A - (Peer Reviewed Journal)
Lam, T.L., Tam, C.C., Stanker, L.H., Cheng, L.W. 2016. Probiotic microorganisms inhibit epithelial cell internalization of botulinum neurotoxin serotype A. Toxins. 8(12):377. doi: 10.3390/toxins8120377.

Identification of botulinum neurotoxin serotype a inhibitors using in vitro cell and oral models of intoxication - (Proceedings)
Cheng, L.W., Tam, C.C., Lam, T.A., Stanker, L.H., Martchenko, M. 2016. Identification of botulinum neurotoxin serotype a inhibitors using in vitro cell and oral models of intoxication. TOXINS 2017 Basic Science and Clinical Aspects of Botulinum and Other Neurotoxins. Meeting Proceedings. Toxicon. Rose Puleo ed. 123 supplement:S16-S17 (25 December 2016).

Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products - (Peer Reviewed Journal)
Hu, L., Ma, L., Zheng, S., He, X., Wang, H., Brown, E., Hammack, T., Zhang, G. 2016. Evaluation of 3M molecular detection system and ANSR pathogen detection system for rapid detection of salmonella from egg products. Poultry Science. 96(5):1410-1418. doi: 10.3382/ps/pew399.

Bithionol blocks pathogenicity of bacterial toxins, ricin, and Zika virus - (Peer Reviewed Journal)
Leonardi, W., Zilbermintz, L., Cheng, L.W., Zozaya, J., Tran, S.H., Elliott, J.H., Polukhina, K., Manasherob, R., Li, A., Chi, X., Gharaibeh, D., Kenny, T., Zamani, R., Soloveva, V., Haddow, A., Nasar, F., Bavari, S., Bassik, M., Cohen, S.N., Levitin, A., Martchenko, M. 2016. Bithionol blocks pathogenicity of bacterial toxins, ricin, and Zika virus. Scientific Reports. 6:34475. doi: 10.1038/srep34475.

Volatile compounds and odor traits of dry-cured ham (Prosciutto crudo) irradiated by electron beam and gamma ray - (Peer Reviewed Journal)
Kong, Q., Yan, W., Yue, L., Chen, Z., Wamg, H., Qi, W., He, X. 2016. Volatile compounds and odor traits of dry-cured ham (Prosciutto crudo) irradiated by electron beam and gamma ray. Journal of Radiation Physics and Chemistry. 130:265-272. doi: 10.1016/j.radphyschem.2016.09.0080969-806X.

Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains - (Peer Reviewed Journal)
Khalil, R., Skinner, C.B., Patfield, S.A., He, X. 2016. Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains. Pathogens and Disease. 74(5):1-11. doi: 10.1093/femspd/ftw037.

Immuno-PCR assay for sensitive detection of proteins in real time - (Book / Chapter)
He, X., Patfield, S.A. 2016. Immuno-PCR assay for sensitive detection of proteins in real time. Methods in Molecular Biology. 1318:139-148. doi: 10.1007/978-1-4939-2742-5_14.