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United States Department of Agriculture

Agricultural Research Service

Related Topics

Bradley Jay Hernlem (Brad)
Foodborne Toxin Detection and Prevention Research
Chemical Engineering

Phone: (510) 559-5937
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY , CA 94710

Projects
Advance the Development of Technologies for Detecting and Determining the Stability and Bioavailability of Toxins that Impact Food Safety and Food Defense
Appropriated (D)
  Accession Number: 430056

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E -
Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E. Toxins. 8(5):150. doi: 10.3390/toxins8050150.
Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1 -
Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1. Journal of Agricultural and Food Chemistry. 80:405-410.
New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes Reprint Icon -
Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.
Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2015. Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin. Toxins. 7(3):835-845. doi: 10.3390/toxins7030835.
Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast Pichia anomala for aflatoxin reduction Reprint Icon -
Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Sarreal, S.L. 2015. Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast Pichia anomala for aflatoxin reduction. World Journal of Microbiology and Biotechnology. doi: 10.1007/s11274-015-1824-3..
Sensitive detection of active Shiga toxin using low cost CCD based optical detector Reprint Icon -
Rasooly, R., Balsam, J., Hernlem, B.J., Rasooly, A.J. 2015. Sensitive detection of active Shiga toxin using low cost CCD based optical detector. Biosensors and Bioelectronics. 68:705-711.
Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells Reprint Icon -
Rasooly, R., Hernlem, B.J. 2014. Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells. Sensors. 14:8869-8876 DOI:10.3390/s140508869.
Rapid detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry -
Ravva, S.V., Hernlem, B.J., Sarreal, C.Z. 2014. Rapid Detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry. Frontiers in Cellular and Infection Microbiology. DOI:10.3389/fcimb.2014.000057.
Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells Reprint Icon -
Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells Reprint Icon -
Rasooly, R., Hernlem, B.J., Friedman, M. 2013. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells. PLoS One. 8(8):e71682. doi:10.1371/journal.pone.0071682.
TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food -
Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.
Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control -
Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Parfitt, D.E., Sarreal, S.L. 2012. Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control. American Society for Microbiology General Meeting. [abstract].
Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract) -
Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.
Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria -
Ravva, S.V., Hernlem, B.J., Sarreal, C.Z., Mandrell, R.E. 2012. Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria. Journal of Environmental Monitoring. 14(2):473-481. doi: 10.1039/clem10573d.
CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A -
Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.
Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells -
Rasooly, R., Do, P.M., Hernlem, B.J. 2011. Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells. Open Journal of Immunology. 1(1):8-14. doi:10.4236/oji.2011.11002.
Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala -
Hua, S.T., Brandl, M., Hernlem, B.J., Eng, J.G., Sarreal, S.L. 2010. Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala. Mycopathologia. Available: http://www.springerlink.com/content/w0204147t65552h8/fulltext.pdf.
Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus -
Hua, S.T., Brandl, M., Hernlem, B.J., Sarreal, S.L. 2010. Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus. American Society for Microbiology General Meeting, San Diego, CA, May 23-27, 2010.
Dihaploid stocks for switchgrass isolated by a screening approach -
Young, H.A., Hernlem, B.J., Anderton, A.L., Lanzatella-Craig, C., Tobias, C.M. 2010. Dihaploid stocks for switchgrass isolated by a screening approach. BioEnergy Research. 3:305-313.
Dual fluorochrome flow cytometric assessment of yeast viability -
Hernlem, B.J., Hua, S.T. 2010. Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiology. 61:57-63.
Development of monoclonal antibodies specific for Ricinus agglutinins Reprint Icon -
Brandon, D.L., Hernlem, B.J. 2009. Development of monoclonal antibodies specific for Ricinus agglutinins. Food and Agricultural Immunology. 20(1):11-22
Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples -
Hernlem, B.J., Ravva, S.V. 2007. Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples. Journal of Environmental Monitoring. 2007(9) 1317-1322.
ELECTROLYTIC DISINFECTION OF ESCHERICHIA COLI AND COLIFORM BACTERIA IN A BATCH CELL WITH DSA ELECTRODES -
Hernlem, B.J. 2006. Electrolytic disinfection of escherichia coli and coliform bacteria in a batch cell with dsa electrodes [Abstract]. ISAC XXIII International Congress. Abstract #229; Poster # P83.
COMBINATION OF CHLORINE-FREE ELECTROLYTIC AND PHOTOCHEMICAL METHODS FOR STERILIZATION OF CONTAMINATED WATERS -
Barashkov, N.N., El Fenni, M., Eisenberg, D., Eisenberg, S., Lam, L., Hernlem, B.J. Combination of chlorine-free electrolytic and photochemical methods for sterilization of contaminated waters [Abstract]. The 232nd ACS National Meeting. Paper ENVR 161.
ELECTROLYTIC DESTRUCTION OF UREA IN DILUTE CHLORIDE SOLUTION USING DSA ELECTRODES IN A RECYCLED BATCH CELL -
Hernlem, B.J. 2005. "Electrolytic Destruction of Urea in Dilute Chloride Solution using DSA Electrodes in a Recycled Batch Cell." Water Research. 39(11):2245-2252
ELECTROFLOTATION OF DAIRY MANURE LAGOON WATER: CHEMICAL & MICROBIOLOGICAL EFFECTS -
Hernlem, B.J. 2004. Electroflotation of dairy manure lagoon water: chemical & microbiological effects. Meeting Abstract.
DISINFECTION AND SOLIDS REMOVAL OF POULTRY CHILLER WATER BY ELECTROFLOTATION -
Tsai, L.-S., Hernlem, B.J., Huxsoll, C.C. 2002. Disinfection and Solids Removal of Poultry Chiller Water by Electroflotation. Journal of Food Science. 67(6):2160-2164.
CHLORINE GENERATION AND DISINFECTION BY ELECTROFLOTATION -
Hernlem, B.J., Tsai, L.-S. 2000. Chlorine Generation and Disinfection by Electroflotation. Journal of Food Science. 65(4):834-837.
COMBINED ELECTROFLOTATION AND DISINFECTION IN FOOD PROCESSING -
Hernlem, B.J., Tsai, L.-S., Huxsoll, C., Robertson, G. 2000. Combined Electroflotation and Disinfection in Food Processing. Paper Number 74-12, presented at the 2000 Institute of Food Technologists Annual Meeting, June 10-14, 2000, Dallas, TX. Available: http://ift.confex.com/ift/2000/techprogram/paper_4502.htm.
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Last Modified: 5/2/2016
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