Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » People & Locations » Rohana Dassanayake

Rohana P Dassanayake
Ruminant Diseases and Immunology Research
Research Molecular Biologist

Phone: (515) 337-7311
Fax:

P.O. Box 70
1920 DAYTON AVE
AMES, IA 50010

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
Intervention Strategies to Prevent and Control Viral Respiratory Pathogens of Ruminants
In-House Appropriated (D)
  Accession Number: 441143
Host-pathogen Interactions and Control Strategies for Bacterial Respiratory Pathogens in Cattle
In-House Appropriated (D)
  Accession Number: 441196
Host-pathogen Interactions and Control Strategies for Bacterial Respiratory Pathogens in Cattle
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442729
Facilitated Expert Gap Analysis and Review of Contagious Bovine Pleuropneumonia (CBPP)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442930
Generation of a Transposon Mutant Library of Mycoplasma Mycoides Subspecies Mycoides
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442932

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Increasing antimicrobial susceptibility of MDR Salmonella with the efflux pump inhibitor 1-(1-Naphthylmethyl)-piperazine Reprint Icon - (Peer Reviewed Journal)
Price, E.D., Dassanayake, R.P., Bearson, S.M. 2023. Increasing antimicrobial susceptibility of MDR Salmonella with the efflux pump inhibitor 1-(1-Naphthylmethyl)-piperazine. Biochemical and Biophysical Research Communications. 668:49-54. https://doi.org/10.1016/j.bbrc.2023.05.035.
Comparative evaluation of antimicrobial activity of human granulysin, bovine and porcine NK-lysins against Shiga toxin-producing Escherichia coli O157:H7 - (Abstract Only)
Identfication of a DRB3*1101-restricted CD4 T cell response against bovine respiratory syncytial virus fusion protein - (Peer Reviewed Journal)
Differential identification of Mannheimia haemolytica genotypes 1 and 2 using colorimetric loop-mediated isothermal amplification Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Clawson, M.L., Tatum, F.M., Briggs, R.E., Kaplan, B.S., Casas, E. 2023. Differential identification of Mannheimia haemolytica genotypes 1 and 2 using colorimetric loop-mediated isothermal amplification. BMC Research Notes. 16:4. https://doi.org/10.1186/s13104-023-06272-8.
NK-lysin antimicrobial peptide-functionalized nanoporous alumina membranes as biosensors for label-free bacterial endotoxin detection Reprint Icon - (Peer Reviewed Journal)
Jiang, N., Shrotriya, P., Dassanayake, R.P. 2022. NK-lysin antimicrobial peptide-functionalized nanoporous alumina membranes as biosensors for label-free bacterial endotoxin detection. Biochemical and Biophysical Research Communications. 636:18-23. https://doi.org/10.1016/j.bbrc.2022.10.097.
MicroRNA profiles for different tissues from calves challenged with Mycoplasma bovis or challenged with Mycoplasma bovis and bovine viral diarrhea virus Reprint Icon - (Peer Reviewed Journal)
Casas, E., Falkenberg, S.M., Dassanayake, R.P., Register, K.B., Neill, J.D. 2022. MicroRNA profiles for different tissues from calves challenged with Mycoplasma bovis or challenged with Mycoplasma bovis and bovine viral diarrhea virus. PLoS ONE. 17(7): Article e0271581. https://doi.org/10.1371/journal.pone.0271581.
Genome sequence and experimental infection of calves with bovine gammaherpesvirus 4 (BoHV-4) Reprint Icon - (Peer Reviewed Journal)
Bauemann, F.V., Falkenberg, S.M., Martins, M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F., Silveria, S., Palmer, M., Buysse, A., Mohr, A., Flores, E., Diel, D. 2022. Genome sequence and experimental infection of calves with bovine gammaherpesvirus 4 (BoHV-4). Journal of General Virology. 167(8):1659-1668. https://doi.org/10.1007/s00705-022-05486-8.
A serosurvey for ruminant pestivirus exposure conducted using sera from stray Mexico origin cattle captured crossing into Southern Texas Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Bauermann, F., Scoles, G.A., Bonilla, D., Dassanayake, R.P. 2022. A serosurvey for ruminant pestivirus exposure conducted using sera from stray Mexico origin cattle captured crossing into Southern Texas. Frontiers in Veterinary Science. 9. Article 821247. https://doi.org/10.3389/fvets.2022.821247.
Comparative study of antibacterial activity and stability of D-enantiomeric and L-enantiomeric bovine NK-lysin peptide NK2A Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Porter, T.J., Samorodnitsky, D., Falkenberg, S.M., Nicholson, E.M., Tatum, F.M., Briggs, R.E., Palmer, M.V., Casas, E. 2022. Comparative study of antibacterial activity and stability of D-enantiomeric and L-enantiomeric bovine NK-lysin peptide NK2A. Biochemical and Biophysical Research Communications. 595:76-81. https://doi.org/10.1016/j.bbrc.2022.01.071.
Vitamin D3 alters macrophage phenotype and endosomal trafficking markers in dairy cattle naturally infected with Mycobacterium avium subsp. paratuberculosis Reprint Icon - (Peer Reviewed Journal)
Wherry, T., Dassanayake, R.P., Bannantine, J.P., Mooyottu, S., Stabel, J.R. 2022. Vitamin D3 alters macrophage phenotype and endosomal trafficking markers in dairy cattle naturally infected with Mycobacterium avium subsp. paratuberculosis. Frontiers in Cellular and Infection Microbiology. Vol.11, article 773938. https://doi.org/10.3389/fcimb.2021.773938.
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates Reprint Icon - (Peer Reviewed Journal)
Mosena, A.S., Falkenberg, S.M., Ma, H., Casas, E., Dassanayake, R.P., Booth, R., De Mia, G., Schweizer, M., Canal, C., Neill, J.D. 2021. Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates. Journal of Virological Methods. 299. Article 114328. https://doi.org/10.1016/j.jviromet.2021.114328.
Bovine NK-lysin peptides exert potent antimicrobial activity against multidrug-resistant Salmonella outbreak isolates Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Atkinson, B.M., Mulls, A.S., Falkenberg, S.M., Nicholson, E.M., Casas, E., Narasimhan, B., Bearson, S.M. 2021. Bovine NK-lysin peptides exert potent antimicrobial activity against multidrug-resistant Salmonella outbreak isolates. Nature Scientific Reports. 11(1). Article 19276. https://doi.org/10.1038/s41598-021-98860-6.
Protection against Mycoplasma bovis infection in calves following intranasal vaccination with modified-live Mannheimia haemolytica expressing Mycoplasma antigens Reprint Icon - (Peer Reviewed Journal)
Briggs, R.E., Billing, S.R., Boatwright Jr, W.D., Chriswell, B.O., Casas, E., Dassanayake, R.P., Palmer, M.V., Register, K.B., Tatum, F.M. 2021. Protection against Mycoplasma bovis infection in calves following intranasal vaccination with modified-live Mannheimia haemolytica expressing Mycoplasma antigens. Microbial Pathogenesis. 161. Article 105159. https://doi.org/10.1016/j.micpath.2021.105159.
Evaluation of antigenic comparisons among BVDV isolates as it relates to humoral and cell mediated immunological measures Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Terhaar, B., Ridpath, J., Neill, J.D., Roth, J. 2021. Evaluation of antigenic comparisons among BVDV isolates as it relates to humoral and cell mediated immunological measures. Frontiers in Veterinary Science. 8. Article 685114. https://doi.org/10.3389/fvets.2021.685114.
Bovine NK-lysin-derived peptides are bactericidal against Mycobacterium avium subspecies paratuberculosis Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Wherry, T.L., Falkenberg, S.M., Reinhardt, T.A., Casas, E., Stabel, J.R. 2021. Bovine NK-lysin-derived peptides are bactericidal against Mycobacterium avium subspecies paratuberculosis. Veterinary Research. 52. Article 11. https://doi.org/10.1186/s13567-021-00893-2.
Virus strain influenced the interspecies transmission of influenza D virus between calves and pigs Reprint Icon - (Peer Reviewed Journal)
Kaplan, B.S., Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Velayudhan, B., Li, F., Vincent, A.L. 2020. Virus strain influenced the interspecies transmission of influenza D virus between calves and pigs. Transboundary and Emerging Diseases. https://doi.org/10.1111/tbed.13943.
Changes in circulating lymphocytes and lymphoid tissue associated with vaccination of colostrum deprived calves Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Palmer, M.V., Silveria, S., Roth, J.A., Gauger, E., Kaiser, T., Guidarini, C., Neill, J.D., Ridpath, J. 2020. Changes in circulating lymphocytes and lymphoid tissue associated with vaccination of colostrum deprived calves. Vaccine. 38(46):7268-7277. https://doi.org/10.1016/j.vaccine.2020.09.046.
Multivariate analysis as a method to evaluate antigenic relationships between BVDV vaccine and field strains Reprint Icon - (Peer Reviewed Journal)
Mosena, A.C., Falkenberg, S.M., Ma, H., Casas, E., Dassanayake, R.P., Watz, P., Canal, C., Neill, J.D. 2020. Multivariate analysis as a method to evaluate antigenic relationships between BVDV vaccine and field strains. Vaccine. 38(36):5764-5772. https://doi.org/10.1016/j.vaccine.2020.07.010.
Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Stasko, J.A., Shircliff, A.L., Lippolis, J.D., Briggs, R.E. 2020. Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation. PLoS One. 15(5):e0233973. https://doi.org/10.1371/journal.pone.0233973.
Measuring CMI responses using the PrimeFlow RNA assay; a new method of evaluating BVDV vaccination response in cattle Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Walz, P., Casas, E., Ridpath, J.F., Roth, J. 2020. Measuring CMI responses using the PrimeFlow RNA assay; a new method of evaluating BVDV vaccination response in cattle. Veterinary Immunology and Immunopathology. 221:110024. https://doi.org/10.1016/j.vetimm.2020.110024.
PAD-bead enrichment enhances detection of PrPSc using real-time quaking-induced conversion Reprint Icon - (Peer Reviewed Journal)
Hwang, S., Dassanayake, R.P., Nicholson, E.M. 2019. PAD-bead enrichment enhances detection of PrPSc using real-time quaking-induced conversion. Bioscience Reports. 12:806. https://doi.org/10.1186/s13104-019-4842-7.
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Silveria, S., Falkenberg, S.M., Dassanayake, R.P., Walz, P.H., Ridpath, J.F., Canal, C.W., Neill, J.D. 2019. In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay. Virology. 536:101-109. https://doi.org/10.1016/j.virol.2019.07.029.
Synthetic bovine NK-lysin-derived peptide (bNK2A) does not require intra-chain disulfide bonds for bactericidal activity Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Nicholson, E.M., Briggs, R.E., Tatum, F.M., Sharma, V.K., Reinhardt, T.A. 2019. Synthetic bovine NK-lysin-derived peptide (bNK2A) does not require intra-chain disulfide bonds for bactericidal activity. PLoS One. 14(6). https://doi.org/10.1371/journal.pone.0218507.
A serosurvey for bovine Influenza D virus exposure from cattle in the United States, 2014-2015 Reprint Icon - (Peer Reviewed Journal)
Silveira, S., Falkenberg, S.M., Kaplan, B.S., Crossley, B., Ridpath, J.F., Bauermann, F.B., Fossler, C.P., Dargatz, D.A., Dassanayake, R.P., Vincent, A.L., Canal, C.W., Neill, J.D. 2019. Serosurvey for influenza D virus exposure in cattle, United States, 2014-2015. Emerging Infectious Diseases. 25(11). https://doi.org/10.3201/eid2511.1902532.
Bovine NK-lysin-derived peptide NK2A demonstrates immunomodulatory activity on cattle leukocytes - (Abstract Only)
Tissue inhibitor of metalloproteinase-1 and interleukin-10 in serum from naïve and scrapie infected sheep Reprint Icon - (Peer Reviewed Journal)
Silva, M.G., Madsen-Bouterse, S.A., Dassanayake, R.P., Mousel, M., Knowles, D.P. 2019. Tissue inhibitor of metalloproteinase-1 and interleukin-10 in serum from naïve and scrapie infected sheep. Veterinary and Animal Science. 7. https://doi.org/10.1016/j.vas.2019.100056.
MicroRNA expression in thymus from calves in a coinfection study challenged with bovine viral diarrhea virus and Mycoplasma bovis - (Abstract Only)
Frequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Walz, P., Casas, E., Neill, J.D., Ridpath, J.F. 2019. Frequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome. Veterinary Immunology and Immunopathology. 207:46-52. https://doi.org/10.1016/j.vetimm.2018.11.015.
Low-volume goat milk transmission of classical scrapie to lambs and goat kids Reprint Icon - (Peer Reviewed Journal)
Madsen-Bouterse, S.A., Highland, M.A., Dassanayake, R.P., Zhuang, D., Schneider, D.A. 2018. Low-volume goat milk transmission of classical scrapie to lambs and goat kids. PLoS One. 13(9):e0204281. https://doi.org/10.1371/journal.pone.0204281.
Experimental infection of calves with bovine gammaherpesvirus 4 - (Abstract Only)
Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Register, K.B., Samorodnitsky, D., Nicholson, E.M., Reinhardt, T.A. 2018. Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis. PLoS One. 13(5):e0197677. https://doi.org/10.1371/journal.pone.0197677.
Serological survey for antibodies against pestiviruses in Wyoming domestic sheep Reprint Icon - (Peer Reviewed Journal)
Silveira, S., Falkenberg, S.M., Elderbrook, M.J., Sondgeroth, K.S., Dassanayake, R.P., Neill, J.D., Ridpath, J.F., Canal, C.W. 2018. Serological survey for antibodies against pestiviruses in Wyoming domestic sheep. Veterinary Microbiology. 219:96-99. https://doi.org/10.1016/j.vetmic.2018.04.019.
Characterization of alpha beta and gamma delta T cell subsets expressing IL-17A in ruminants and swine Reprint Icon - (Peer Reviewed Journal)
Elnaggar, M.M., Abdellrazeq, G.S., Dassanayake, R.P., Fry, L.M., Hulubei, V., Davis, W.C. 2018. Characterization of alpha beta and gamma delta T cell subsets expressing IL-17A in ruminants and swine. Developmental and Comparative Immunology. 85:115-124. https://doi.org/10.1016/j.dci.2018.04.003.
Relationship between viral shedding in the transmission of bovine viral diarrhea virus to naïve calves by direct and indirect exposure routes Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F. 2018. Evaluation of bovine viral diarrhea virus transmission potential to naïve calves by direct and indirect exposure routes. Veterinary Microbiology. 217:144-148. https://doi.org/10.1016/j.vetmic.2018.03.012.
Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis - (Abstract Only)
Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Briggs, R.E., Tatum, F.M., Sacco, R.E. 2017. Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni. PLoS One. 12(8):1-14. doi. 10.1371/journal.pone.0183610.
Improved detection of Bovine Viral Diarrhea Virus in Bovine lymphoid cell lines using PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Silveira, S., Neill, J.D., Ridpath, J.F. 2017. Improved detection of Bovine Viral Diarrhea Virus in Bovine lymphoid cell lines using PrimeFlow RNA assay. American Association of Veterinary Laboratory Diagnosticians. 235:77-81. 10.1016/j.virol.2017.06.032.
Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni - (Abstract Only)
Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F. 2017. Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay. Virology. 509(2017):260-265. doi. 10.1016/j.virol.2017.06.032.
Differential susceptibility of bighorn sheep (Ovis Canadensis) and domestic sheep (Ovis Aries) neutrophils to Mannheimia Haemolytica Leukotoxin is not due to differential expression of cell surface CD18 Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Shanthalingam, S., Liu, W., Casas, E., Srikumaran, S. 2017. Differential susceptibility of bighorn sheep (Ovis Canadensis) and domestic sheep (Ovis Aries) neutrophils to Mannheimia Haemolytica Leukotoxin is not due to differential expression of cell surface CD18. Journal of Wildlife Diseases. 53(3):625-629. doi: 10.7589/2016-11-244.
Morphological changes in cultured bovine lymphoid cell lines associated with bovine viral diarrhea virus (BVDV) single and dual infections with bovine leukemia virus (BLV) - (Abstract Only)
Differential susceptibility of bighorn sheep and domestic sheep neutrophils to Mannheimia haemolytica leukotoxin is not due to differential expression of cell surface CD18 - (Abstract Only)