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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » People & Locations » Rohana Dassanayake

Rohana P Dassanayake
Ruminant Diseases and Immunology Research
Research Molecular Biologist

Phone: (515) 337-7311
Fax:

P.O. Box 70
1920 DAYTON AVE
AMES, IA 50010
Projects
Identification of Disease Mechanisms and Control Strategies for Bacterial Respiratory Pathogens in Ruminants
In-House Appropriated (D)
  Accession Number: 432020
Identification of Disease Mechanisms and Control Strategies for Viral Respiratory Pathogens of Ruminants
In-House Appropriated (D)
  Accession Number: 432021
Intracellular Killing And Biofilm Destruction Of MDR Salmonella By NK-Lysin Encapsulated Nanoparticles
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438266

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Changes in circulating lymphocytes and lymphoid tissue associated with vaccination of colostrum deprived calves - (Peer Reviewed Journal)
Multivariate analysis as a method to evaluate antigenic relationships between BVDV vaccine and field strains Reprint Icon - (Peer Reviewed Journal)
Mosena, A.C., Falkenberg, S.M., Ma, H., Casas, E., Dassanayake, R.P., Watz, P., Canal, C., Neill, J.D. 2020. Multivariate analysis as a method to evaluate antigenic relationships between BVDV vaccine and field strains. Vaccine. 38(36):5764-5772. https://doi.org/10.1016/j.vaccine.2020.07.010.
Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Stasko, J.A., Shircliff, A.L., Lippolis, J.D., Briggs, R.E. 2020. Identification of a reliable fixative solution to preserve complex architecture of bacterial biofilms for scanning electron microscopy evaluation. PLoS One. 15(5):e0233973. https://doi.org/10.1371/journal.pone.0233973.
Measuring CMI responses using the PrimeFlow RNA assay; a new method of evaluating BVDV vaccination response in cattle Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Walz, P., Casas, E., Ridpath, J.F., Roth, J. 2020. Measuring CMI responses using the PrimeFlow RNA assay; a new method of evaluating BVDV vaccination response in cattle. Veterinary Immunology and Immunopathology. 221:110024. https://doi.org/10.1016/j.vetimm.2020.110024.
PAD-bead enrichment enhances detection of PrPSc using real-time quaking-induced conversion Reprint Icon - (Peer Reviewed Journal)
Hwang, S., Dassanayake, R.P., Nicholson, E.M. 2019. PAD-bead enrichment enhances detection of PrPSc using real-time quaking-induced conversion. Bioscience Reports. 12:806. https://doi.org/10.1186/s13104-019-4842-7.
In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Silveria, S., Falkenberg, S.M., Dassanayake, R.P., Walz, P.H., Ridpath, J.F., Canal, C.W., Neill, J.D. 2019. In vitro method to evaluate virus competition between BVDV-1 and BVDV-2 strains using the PrimeFlow RNA assay. Virology. 536:101-109. https://doi.org/10.1016/j.virol.2019.07.029.
Synthetic bovine NK-lysin-derived peptide (bNK2A) does not require intra-chain disulfide bonds for bactericidal activity Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Nicholson, E.M., Briggs, R.E., Tatum, F.M., Sharma, V.K., Reinhardt, T.A. 2019. Synthetic bovine NK-lysin-derived peptide (bNK2A) does not require intra-chain disulfide bonds for bactericidal activity. PLoS One. 14(6). https://doi.org/10.1371/journal.pone.0218507.
A serosurvey for bovine Influenza D virus exposure from cattle in the United States, 2014-2015 Reprint Icon - (Peer Reviewed Journal)
Silveira, S., Falkenberg, S.M., Kaplan, B.S., Crossley, B., Ridpath, J.F., Bauermann, F.B., Fossler, C.P., Dargatz, D.A., Dassanayake, R.P., Vincent, A.L., Canal, C.W., Neill, J.D. 2019. Serosurvey for influenza D virus exposure in cattle, United States, 2014-2015. Emerging Infectious Diseases. 25(11). https://doi.org/10.3201/eid2511.1902532.
Bovine NK-lysin-derived peptide NK2A demonstrates immunomodulatory activity on cattle leukocytes - (Abstract Only)
Tissue inhibitor of metalloproteinase-1 and interleukin-10 in serum from naïve and scrapie infected sheep Reprint Icon - (Peer Reviewed Journal)
Silva, M.G., Madsen-Bouterse, S.A., Dassanayake, R.P., Mousel, M., Knowles, D.P. 2019. Tissue inhibitor of metalloproteinase-1 and interleukin-10 in serum from naïve and scrapie infected sheep. Veterinary and Animal Science. 7. https://doi.org/10.1016/j.vas.2019.100056.
MicroRNA expression in thymus from calves in a coinfection study challenged with bovine viral diarrhea virus and Mycoplasma bovis - (Abstract Only)
Frequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Walz, P., Casas, E., Neill, J.D., Ridpath, J.F. 2019. Frequency of bovine viral diarrhea virus detected in subpopulations of peripheral blood mononuclear cells in persistently infected animals and health outcome. Veterinary Immunology and Immunopathology. 207:46-52. https://doi.org/10.1016/j.vetimm.2018.11.015.
Low-volume goat milk transmission of classical scrapie to lambs and goat kids Reprint Icon - (Peer Reviewed Journal)
Madsen-Bouterse, S.A., Highland, M.A., Dassanayake, R.P., Zhuang, D., Schneider, D.A. 2018. Low-volume goat milk transmission of classical scrapie to lambs and goat kids. PLoS One. 13(9):e0204281. https://doi.org/10.1371/journal.pone.0204281.
Experimental infection of calves with bovine gammaherpesvirus 4 - (Abstract Only)
Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Register, K.B., Samorodnitsky, D., Nicholson, E.M., Reinhardt, T.A. 2018. Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis. PLoS One. 13(5):e0197677. https://doi.org/10.1371/journal.pone.0197677.
Serological survey for antibodies against pestiviruses in Wyoming domestic sheep Reprint Icon - (Peer Reviewed Journal)
Silveira, S., Falkenberg, S.M., Elderbrook, M.J., Sondgeroth, K.S., Dassanayake, R.P., Neill, J.D., Ridpath, J.F., Canal, C.W. 2018. Serological survey for antibodies against pestiviruses in Wyoming domestic sheep. Veterinary Microbiology. 219:96-99. https://doi.org/10.1016/j.vetmic.2018.04.019.
Characterization of alpha beta and gamma delta T cell subsets expressing IL-17A in ruminants and swine Reprint Icon - (Peer Reviewed Journal)
Elnaggar, M.M., Abdellrazeq, G.S., Dassanayake, R.P., Fry, L.M., Hulubei, V., Davis, W.C. 2018. Characterization of alpha beta and gamma delta T cell subsets expressing IL-17A in ruminants and swine. Developmental and Comparative Immunology. 85:115-124. https://doi.org/10.1016/j.dci.2018.04.003.
Relationship between viral shedding in the transmission of bovine viral diarrhea virus to naïve calves by direct and indirect exposure routes Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F. 2018. Evaluation of bovine viral diarrhea virus transmission potential to naïve calves by direct and indirect exposure routes. Veterinary Microbiology. 217:144-148. https://doi.org/10.1016/j.vetmic.2018.03.012.
Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis - (Abstract Only)
Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Falkenberg, S.M., Briggs, R.E., Tatum, F.M., Sacco, R.E. 2017. Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni. PLoS One. 12(8):1-14. doi. 10.1371/journal.pone.0183610.
Improved detection of Bovine Viral Diarrhea Virus in Bovine lymphoid cell lines using PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Silveira, S., Neill, J.D., Ridpath, J.F. 2017. Improved detection of Bovine Viral Diarrhea Virus in Bovine lymphoid cell lines using PrimeFlow RNA assay. American Association of Veterinary Laboratory Diagnosticians. 235:77-81. 10.1016/j.virol.2017.06.032.
Antimicrobial activity of bovine NK-lysin-derived peptides on bovine respiratory pathogen Histophilus somni - (Abstract Only)
Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay Reprint Icon - (Peer Reviewed Journal)
Falkenberg, S.M., Dassanayake, R.P., Neill, J.D., Ridpath, J.F. 2017. Improved detection of bovine viral diarrhea virus in bovine lymphoid cell lines using PrimeFlow RNA assay. Virology. 509(2017):260-265. doi. 10.1016/j.virol.2017.06.032.
Differential susceptibility of bighorn sheep (Ovis Canadensis) and domestic sheep (Ovis Aries) neutrophils to Mannheimia Haemolytica Leukotoxin is not due to differential expression of cell surface CD18 Reprint Icon - (Peer Reviewed Journal)
Dassanayake, R.P., Shanthalingam, S., Liu, W., Casas, E., Srikumaran, S. 2017. Differential susceptibility of bighorn sheep (Ovis Canadensis) and domestic sheep (Ovis Aries) neutrophils to Mannheimia Haemolytica Leukotoxin is not due to differential expression of cell surface CD18. Journal of Wildlife Diseases. 53(3):625-629. doi: 10.7589/2016-11-244.
Morphological changes in cultured bovine lymphoid cell lines associated with bovine viral diarrhea virus (BVDV) single and dual infections with bovine leukemia virus (BLV) - (Abstract Only)
Differential susceptibility of bighorn sheep and domestic sheep neutrophils to Mannheimia haemolytica leukotoxin is not due to differential expression of cell surface CD18 - (Abstract Only)