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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Ruminant Diseases and Immunology Research » Research » Publications at this Location » Publication #403195

Research Project: Genomic and Mitigation Strategies to Control Mastitis

Location: Ruminant Diseases and Immunology Research

Title: Genome Sequence of a Multidrug-Resistant Pseudomonas aeruginosa Strain Isolated from a Dairy Cow That was Nonresponsive to Antibiotic Treatment

Author
item Dassanayake, Rohana
item Ma, Hao
item Casas, Eduardo
item LIPPOLIS, JOHN

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/17/2023
Publication Date: 8/15/2023
Citation: Dassanayake, R.P., Ma, H., Casas, E., Lippolis, J.D. 2023. Genome Sequence of a Multidrug-Resistant Pseudomonas aeruginosa Strain Isolated from a Dairy Cow That was Nonresponsive to Antibiotic Treatment. Microbiology Resource Announcements. Article e002892. https://doi.org/10.1128/MRA.00289-23.
DOI: https://doi.org/10.1128/MRA.00289-23

Interpretive Summary: Pseudomonas aeruginosa is an opportunistic pathogen and it can cause acute and chronic mastitis in cattle. Pseudomonas species are environmental pathogens and can be found in humid areas such as soiled beddings, manure, contaminated water, and milking parlors. Here in this study, whole genome of a multidrug resistant P. aeruginosa strain, which was isolated from a naturally occurring case of chronic mastitis, was sequenced. Presence of multiple antibiotic resistance genes in its' genome explained the lack of initial response to antibiotic treatment and should assist us to develop future treatment plans.

Technical Abstract: We report the complete genome sequence of a multidrug-resistant Pseudomonas aeruginosa strain isolated from a Holstein cow with chronic mastitis. The assembled genome contained 108 contigs with an N50 of 130,886 bp, 66.03%, GC content, 6,214 protein-coding genes, 64 RNA genes, 88 pseudogenes, and six antibiotic resistant genes.