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United States Department of Agriculture

Agricultural Research Service

Related Topics

Phil H Purdy
Plant and Animal Genetic Resources Preservation
Physiology

Phone: (970) 492-7512
Fax: (970) 492-7505

1111 SOUTH MASON STREET
1111 SOUTH MASON STREET
NCGRP
FORT COLLINS , CO 80526

Projects
National Animal Germplasm Program
Appropriated (D)
  Accession Number: 423549
Development of a Non-Surgical Sheep Artificial Insemination Technique Utilizing Frozen-Thawed Semen
Nonfunded Cooperative Agreement (N)
  Accession Number: 424372
Development of a non-Surgical Artificial Insemination Technique for Use with Sheep and Frozen-Thawed Ram Semen
Nonfunded Cooperative Agreement (N)
  Accession Number: 425638
Development of a Non-Surgical Sheep Artificial Insemination Technique Utilizing Frozen-Thawed Semen
Nonfunded Cooperative Agreement (N)
  Accession Number: 425660
Development of a Sheep Artificial Insemination Procedure Utilizing Frozen-Thawed Semen and a Non-Surgical Insemination Technique
Nonfunded Cooperative Agreement (N)
  Accession Number: 425661
Standardization of Sperm Evaluation Across Gene Banks
Specific Cooperative Agreement (S)
  Accession Number: 429428

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Modification of trout sperm membranes associated with activation and cryopreservation. Implications for fertilizing potential -
Purdy, P.H., Barbosa, E.A., Praamsma, C.J., Schisler, G.J. 2016. Modification of trout sperm membranes associated with activation and cryopreservation. Implications for fertilizing potential. Cryobiology. 73:73-79.
Biobanking genetic resources: Challenges and implementation at the USDA National Animal Germplasm Program -
Purdy, P.H., Wilson, C.S., Spiller, S.F., Blackburn, H.D. 2015. Biobanking genetic resources: Challenges and implementation at the USDA National Animal Germplasm Program. Reproduction, Fertility and Development. 28:1072-1078.
Enhance of the estrus and ovulation sychronism in ewes using protocols with progesterone -
Nathalia, M.H., Brasil, O.O., Silva, P.C., Saavedra, C.A., Padilha Da Silva, J., Azevedo, H.C., Paiva, S.R., Purdy, P.H., Ramos, A.F. 2015. Enhance of the estrus and ovulation sychronism in ewes using protocols with progesterone. Meeting Abstract. 10º Simpósio de Recursos Genéticos para a América Latina e o Caribe, Oct 26-28, 2015, Bento Gonçalves, RS, Brazil.
Utilizing cattle genetic trends to evaluate the robust nature of gene bank collections -
Blackburn, H.D., Wilson, C.S., Paiva, S., Spiller, S.F., Purdy, P.H. 2015. Utilizing cattle genetic trends to evaluate the robust nature of gene bank collections. Meeting Abstract. ADSA-ASAS Joint Annual Meeting Orlando, FL July 12-16, 2015.
An update on USDA swine gene banking -
Purdy, P.H., Blackburn, H.D., Manahan, T.J., Spiller, S.F., Wilson, C.S. 2015. An update on USDA swine gene banking. Meeting Abstract. VIII International Conference on Boar Semen Preservation, Champaign, IL August 9-12, 2015. Vol 50:112-113.
Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment -
Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Krisher, R.L., Knox, R.V., Rodriguez-Zas, S.L., Miller, D.J. 2015. Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment. Journal of Andrology. 3:558-568.
A preliminary report on the impact of cryopreservation diluent on ram sperm physiology and cervical artificial insemination -
Purdy, P.H., Spiller, S.F., Rice, S.D., Koeoke, K.L., Mcguire, E.M., Mcguire, K.E., Stobart, S., Blackburn, H.D. 2014. A preliminary report on the impact of cryopreservation diluent on ram sperm physiology and cervical artificial insemination. Symposium Proceedings. Western Section American Society of Animal Science, June 25-27, 2014, San Angelo, Texas.
Reproduction -
Inskeep, K., Keisler, D.H., Purdy, P.H. 2016. Reproduction. Pp. 1000-1044. In: American Sheep Industry Association Inc (eds.). Sheep Production Handbook. Volume 8. Book Chapter. ADS Publishing, Fort Collins, Colorado.
Membrane modification strategies for cryopreservation. pp 337-342. In: Willem F. Wolkers and Harriette Oldenhof (eds.). Cryopreservation and Freeze-Drying Protocls. 3rd ed.The Lab Protocol Series Methods in Molecular Biology. -
Purdy, P.H., Graham, J.K. 2014. Membrane modification strategies for cryopreservation. In: Wolkers, W.F., Oldenhof, H., editors. Cryopreservation and Freeze-Drying Protocls. Third Edition. The Lab Protocol Series Methods in Molecular Biology. Book Chapter. New York: Springer. p. 337-342.
Fertility prediction of frozen boar sperm using novel and conventional analyses -
Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Rodriguez_Zas, S.L., Krisher, R.L., Knox, R.V., Miller, D.J. 2014. Fertility prediction of frozen boar sperm using novel and conventional analyses. Meeting Abstract. Society for the Study of Reproduction, Grand Rapids, MI, July 19-23, 2014.
Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success -
Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Krisher, R.L., Knox, R.V., Miller, D.J. 2014. Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success. Theriogenology. 82:266-273.
Rooster sperm plasma membrane protein and phospholipid organization and reorganization attributed to cooling and cryopreservation -
Purdy, P.H., Spiller, S.F., Blackburn, H.D. 2013. Rooster sperm plasma membrane protein and phospholipid organization and reorganization attributed to cooling and cryopreservation. Meeting Abstract. Annual Meeting of the Society for Cryobiology, Bethesda, MD, July 28-31, 2013.
Post-thaw motility of frozen boar sperm does not predict success with in vitro fertilization -
Daigneault, B.W., Mcnamara, K.A., Purdy, P.H., Knox, R.V., Krisher, R.L., Miller, D.J. 2013. Post-thaw motility of frozen boar sperm does not predict success with in vitro fertilization. Meeting Abstract. Society for the Study of Reproduction, Montreal, Quebec, Canada, July 22-26, 2013.
Effect of storage time on the viability of cryopreserved bovine spermatozoa -
Gallegos, A.I., Ericsson, S.A., Blackburn, H.D., Spiller, S.F., Warnock, B.J., Meador, M.K., Smith, M.W., Purdy, P.H. 2013. Effect of storage time on the viability of cryopreserved bovine spermatozoa. Meeting Abstract. American Society of Animal Science, Indianapolis, IN, July 8-12, 2013.
Current biotechnologies and future possibilities in preservation of animal germplasm -
Purdy, P.H. 2013. Current biotechnologies and future possibilities in preservation of animal germplasm. Meeting Abstract. The 2nd International Symposium on Plant Cryopreservation, Fort Collins, CO Aug 11-14, 2013.
A simple vitrification method for cryobanking avian testicular tissue -
Liu, J., Cheng, K.M., Purdy, P.H., Silversides, F.G. 2012. A simple vitrification method for cryobanking avian testicular tissue. Poultry Science. 91:3209-3213.
Effects of alternative cryoprotectants, diluents, straw size and cholesterol addition on cryopreserved rooster sperm -
Tarvis, K.M., Purdy, P.H., Graham, J.K. 2012. Effects of alternative cryoprotectants, diluents, straw size and cholesterol addition on cryopreserved rooster sperm. Meeting Abstract. International Congress on Animal Reproduction, Vancouver, BC, Canada, July 29-August 2, 2012.
185 Cost effectiveness of maintaining research chicken populations in situ or with cryopreserved semen or ovaries -
Silversides, F.G., Purdy, P.H., Blackburn, H.D. 2012. 185 Cost effectiveness of maintaining research chicken populations in situ or with cryopreserved semen or ovaries. Meeting Abstract. Poultry Science Association, Athens, GA, July 9-12.
Comparative costs of programmes to conserve chicken genetic variation based on maintaining living populations or storing cryopreserved material -
Silversides, F., Purdy, P.H., Blackburn, H.D. 2013. Comparative costs of programmes to conserve chicken genetic variation based on maintaining living populations or storing cryopreserved material. British Poultry Science. 53:599-607.
Boar sperm quality in lines of pigs selected for either ovulation rate or uterine capacity Reprint Icon -
Freking, B.A., Purdy, P.H., Spiller, S.F., Welsh, C.S., Blackburn, H.D. 2012. Boar sperm quality in lines of pigs selected for either ovulation rate or uterine capacity. Journal of Animal Science. 90(8):2515-2523.
Changing rooster sperm membranes to facilitate cryopreservation -
Graham, J.K., Travis, K.M., Purdy, P.H. 2012. Changing rooster sperm membranes to facilitate cryopreservation. Meeting Abstract. International Embryo Transfer Society, Phoenix, AZ. January 10, 2012.
Cryoconservation of animal genetic resources. Animal Production and Health Guidelines No. 12 -
Blackburn, H.D., Godke, R., Purdy, P.H., Hiemstra, S., Woelders, H., Mariante, A., Pizzi, F., Boettcher, P. 2012. Cryoconservation of animal genetic resources. Animal Production and Health Guidelines No. 12. Complete Book. FAO, Rome p. 222.
Genetic Structure and Diversity among U.S. sheep breeds: Identification of the major gene pools. -
Blackburn, H.D., Paiva, S.R., Wildeus, S., Getz, W., Stobart, R., Bixby, D., Purdy, P.H., Welsh, C.S., Spiller, S.F., Brown, M.A. 2011. Genetic Structure and Diversity among U.S. sheep breeds: Identification of the major gene pools. Journal of Animal Science. 89:2336-2348.
Sperm quality assessment in mammals by flow cytometry In: T.R. Tiersch and C.C. Green (eds.) Cryopreservation in Aquatic Species, 2nd Edition. World Aquaculture Society, Baton Rouge, LA. -
Purdy, P.H. 2011. Sperm quality assessment in mammals by flow cytometry pp. 219-226. In: T.R. Tiersch and C.C. Green (eds.) Cryopreservation in Aquatic Species, 2nd Edition. World Aquaculture Society, Baton Rouge, LA.
Effect of number of motile frozen-thawed boar sperm and number of fixed-time inseminations on fertility in estrous-synchronized gilts -
Spencer, K.W., Purdy, P.H., Blackburn, H.D., Spiller, S.F., Stewart, T.S., Knox, R.V. 2010. Effect of number of motile frozen-thawed boar sperm and number of fixed-time inseminations on fertility in estrous-synchronized gilts. Animal Reproduction Sciences. 121:259-266.
Reproductive biotechnologies and management of animal genetic resources -
Blackburn, H.D., Purdy, P.H. 2010. Reproductive biotechnologies and management of animal genetic resources. XIIIth European Poultry Conference, Tours France, August 23-27, 2010. Meeting Proceedings.
Implications of the pH and temperature of diluted, cooled boar semen on fresh and frozen-thawed sperm motility characteristics -
Purdy, P.H., Tharp, N., Stewart, T., Spiller, S.F., Blackburn, H.D. 2010. Implications of the pH and temperature of diluted, cooled boar semen on fresh and frozen-thawed sperm motility characteristics. Theriogenology. 74:1304-1310.
Evaluation of Glycerol Removal Techniques, Cryoprotectants, and Insemination Methods for Cryopreserving Rooster Sperm with Implications for Breed and/or Line Regeneration -
Purdy, P.H., Song, Y., Silversides, F.G., Blackburn, H.D. 2009. Evaluation of Glycerol Removal Techniques, Cryoprotectants, and Insemination Methods for Cryopreserving Rooster Sperm with Implications for Breed and/or Line Regeneration. Poultry Science. 88:2184-2191
Inseminating fresh or cryopreserved semen for maximum efficiency: implications for gene banks and industry -
Blackburn, H.D., Silversides, F., Purdy, P.H. 2009. Inseminating fresh or cryopreserved semen for maximum efficiency: implications for gene banks and industry. Poultry Science. 88:2192-2198.
The fertility of ram sperm held for 24 hours at 5 ºC prior to cryopreservation -
Purdy, P.H., Moce, E., Stobart, R., Murdoch, W.J., Moss, G.E., Larson, B., Ramsey, S., Graham, J.K., Blackburn, H.D. 2009. The fertility of ram sperm held for 24 hours at 5 ºC prior to cryopreservation. Animal Reproduction Sciences. 118:231-235.
Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival -
Moce, E., Purdy, P.H., Graham, J.K. 2009. Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival. Animal Reproduction Sciences. 118:236-247.
Conservation of Swine Genetics by the National Animal Germplasm Program (NAGP) after One Decade Reprint Icon -
Blackburn, H.D., Stewart, T., Purdy, P.H. 2009. Conservation of Swine Genetics by the National Animal Germplasm Program (NAGP) after One Decade. National Hog Farmer.
Investigation of a novel non-surgical method of artificial insemination for sheep -
PPurdy, P.H., Blackburn, H.D., Larson, B., Stobart, R. 2009. Investigation of a novel non-surgical method of artificial insemination for sheep. Western section of Amercan Society of animal Science, Fort Collins, CO, June 16-18, 2009.
Influence of pre-cryopreservation pH and temperature on boar semen quality -
Tharp, N.E., Purdy, P.H., Blackburn, H.D., Spiller, S.F., Stewart, T. 2009. Influence of pre-cryopreservation pH and temperature on boar semen quality. Midwest Section American Society of Animal Science, Des Moines, IA, March 16-18, 2008.
Evaluation of new techniques to enhance the use of A.I. on farm. -
PPurdy, P.H., Stobart, R.H., Larson, B., Blackburn, H.D. 2009. Evaluation of new techniques to enhance the use of A.I. on farm. American Sheep Industry Association Annual Conference, San Diego, CA, January 21-24, 2009.
Effect of number of motile sperm and number of inseminations using frozen boar semen on fertility in gilts -
Spencer, K., Purdy, P.H., Blackburn, H.D., Spiller, S.F., Welsh, C.S., Stewart, T., Breen, S., Taibl, J., Yantis, B., Knox, R. 2008. Effect of number of motile sperm and number of inseminations using frozen boar semen on fertility in gilts. National Hog Farmer. http://nationalhogfarmer.com/genetics-reproduction/artificial-insemination/1215-boar-semen-use/.
Perspectives on cryopreservation, quality control, and artificial insemination of frozen-thawed boar sperm in a national repository -
Purdy, P.H., Knox, R., Singleton, W., Spencer, K., Spiller, S.F., Stewart, T., Tharp, N., Welsh, C.S., Blackburn, H.D. 2008. Perspectives on cryopreservation, quality control, and artificial insemination of frozen-thawed boar sperm in a national repository. Symposium Proceedings. Midwest Boar Stud Managers Conference, Saint Louis, MO August 7-8, 2008.
Ubiquitination and its influence in boar sperm physiology and cryopreservation Reprint Icon -
Purdy, P.H. 2008. Ubiquitination and its influence in boar sperm physiology and cryopreservation. Theriogenology 70:818-826.
Swine gene banking: A quality control perspective on collection, and analysis of samples for a national repository Reprint Icon -
Purdy, P.H. 2008. Gene banking: A quality control perspective on collection, and analysis of samples for a national repository. Theriogenology. 10.1016/j.theriogenology.2008.06.012
Boar fertility and semen quality characteristics in lines of pigs selected for component traits of female reproduction Reprint Icon -
Freking, B.A., Purdy, P.H., Welsh, C.S., Spiller, S.F., Blackburn, H.D. 2008. Boar fertility and semen quality characteristics in lines of pigs selected for component traits of female reproduction [abstract]. Journal of Animal Science Supplement. 86(E-Suppl. 2):208-209. Abstract #W50.
Detection of boar sperm plasma membrane protein using Rhodamine 640; implications for cryobiology and physiology -
Purdy, P.H. 2008. Detection of boar sperm plasma membrane protein using Rhodamine 640; implications for cryobiology and physiology. American Society of Andrology Meeting, Albuquerque, New Mexico, April 2008. Pp. 49.
Gene banking: A quality control perspective on collection, and analysis of samples for a national repository -
Purdy, P.H. 2007. Gene banking: A quality control perspective on collection, and analysis of samples for a national repository. Theriogenology. 6th International Conference on Boar Semen Preservation, Alliston, Ontario, Canada, August 11-15, 2007.
Semen cryopreservation research seeks higher fertility rates in rainbow trout -
Silverstein, J.T., Purdy, P.H., Blackburn, H.D. 2007. Semen cryopreservation research seeks higher fertility rates in rainbow trout. Global Aquaculture Advocate. 10(2):84.
Preserving Ram Semen: New Science Shows it can be Stored for up to Two Days Before Freezing -
Purdy, P.H. 2007. Preserving Ram Semen: New Science Shows it can be Stored for up to Two Days Before Freezing. Sheep! Magazine. p.40-41.
Relationship of flow cytometric evaluations of cryopreserved rainbow trout Oncorhynchus mykiss sperm with fertility -
Purdy, P.H., Blackburn, H.D., Silverstein, J. 2007. Relationship of flow cytometric evaluations of cryopreserved rainbow trout Oncorhynchus mykiss sperm with fertility. Aquaculture 2007 Annual Meeting. p 734.
A Method for Cryopreserving Chicken Primordial Germ Cells -
Moore, D.T., Purdy, P.H., Blackburn, H.D. 2006. A Method for Cryopreserving Chicken Primordial Germ Cells. Poultry Science. 85:1784–1790.
UBIQUITINATION OF BOAR SPERM: WHAT DOES THIS REVEAL ABOUT SPERM PHYSIOLOGY? -
Purdy, P.H. 2006. Ubiquitination of boar sperm: what does this reveal about sperm physiology? Annual Meeting of the Society for the Study of Reproduction, Omaha, NE, July 29-August 1, 2006. p.189.
VARIATION IN SPERM CELL QUALITY IN CRYOPRESERVED BOAR SEMEN -
Stewart, T., Gunsett, F., Welsh, C.S., Spiller, S.F., Purdy, P.H., Blackburn, H.D. 2006. Variation in sperm cell quality in cryopreserved boar semen. 8th World Congress of Genetics Applied in Livestock Production. 11(23):1-4.
THE POST-THAW QUALITY OF RAM SPERM HELD FOR 0 TO 48 HOURS AT 5°C PRIOR TO CRYOPRESERVATION -
Purdy, P.H. 2006. The post-thaw quality of ram sperm held for 0 to 48 hours at 5°c prior to cryopreservation. Animal Reproduction Sciences. 93:114-123.
THE FLUIDITY OF CHINESE HAMSTER OVARY CELL AND BULL SPERM MEMBRANES AFTER CHOLESTEROL ADDITION -
Purdy, P.H., Fox, M.H., Graham, J.K. 2005. The fluidity of chinese hamster ovary cell and bull sperm membranes after cholesterol addition. Cryobiology. 51:102-112.
CHOLESTEROL-LOADED CYCLODEXTRIN IMPROVES POST-THAW GOAT SPERM MOTILITY -
Barrera-Compean, M.H., Purdy, P.H., Dzakuma, J.M., Newton, G.R., Nuti, L.C. 2005. Cholesterol-loaded cyclodextrin improves post-thaw goat sperm motility. Journal of Animal Science. 2005. 83 (Supl 1):153.
A REVIEW ON GOAT SPERM CRYOPRESERVATION -
Purdy, P.H. 2005. A review on goat sperm cryopreservation. Small Ruminant Research.
A REVIEW ON GOAT SPERM CRYOPRESERVATION -
Purdy, P.H. 2006. A review on goat sperm cryopreservation. Small Ruminant Research. 63:215-225.
EFFECTS OF HOLDING TIME PRIOR TO FREEZING ON THE MOTILITY, VIABILITY AND MEMBRANE BINDING ABILITY OF RAM SPERM -
Purdy, P.H. 2004. Effects of holding time prior to freezing on the motility, viability and membrane binding ability of ram sperm. 2004 Joint Annual Meeting of the American Dairy Science Association, American Society of Animal Science and Poultry Science Association, July 26-28, 2004, St. Louis, Missouri. Journal of Animal Science. 82(Suppl 1):367.
EFFECT OF ADDING CHOLESTEROL TO BULL SPERM MEMBRANES ON SPERM CAPACITATION, THE ACROSOME REACTION, AND FERTILITY -
Purdy, P.H., Graham, J.K. 2004. Effect of adding cholesterol to bull sperm membranes on sperm capacitation, the acrosome reaction, and fertility. Biology of Reproduction. 71:522-527.
SPANISH BUCK SS-B INHIBIN/ACTIVIN (INHBB) MICROSATELLITE POLYMORPHISMS -
Realivasquez, R., Ericsson, S.A., Spiller, S.F., Campbell, W.T., Sternes, K.L., Purdy, P.H., Blackburn, H.D., Dzakuma, J.M. 2004. Spanish buck ss-b inhibin/activin (INHBB) microsatellite polymorphisms. American Society of Animal Science/American Society of Dairy Science/Poultry Science Association Annual Meeting, July 25-29, 2004, St. Louis, Missouri. Journal of Animal Science Supplement 2004. 82(Suppl 1): 377-378.
INHA MICROSATELLITE POLYMORPHISMS IN ANGORA BUCKS -
Spiller, S.F., Ericsson, S.A., Realivasquez, R., Campbell, W.T., Sterns, K.L., Purdy, P.H., Blackburn, H.D., Dzakuma, J.M. 2004. INHA microsatellite polymorphisms in angora bucks. American Society of Animal Science/American Society of Dairy Science/Poultry Science Association Annual Meeting, July 25-29, 2004, St. Louis, Missouri. Journal of Animal Science 2004. 82(Suppl 1):378.
EFFECTS OF THE FLAVONOIDS, SILIBININ AND CATECHIN, ON THE MOTILITY OF EXTENDED COOLED CAPRINE SPERM -
Purdy, P.H., Ericsson, S.A., Dodson, R.E., Sternes, K.L., Garner, D.L. 2004. Effects of the flavonoids, silibinin and catechin, on the motility of extended cooled caprine sperm. Small Ruminant Research. 55:239-243.
EFFECT OF CHOLESTEROL-LOADED CYCLODEXTRIN ON THE CRYOSURVIVAL OF BULL SPERM -
Purdy, P.H., Graham, J.K. 2004. Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm. Cryobiology. 48:36-45.
CURRENT STATUS OF CRYOPRESERVING GOAT SEMEN -
Purdy, P.H. 2003. Current status of cryopreserving goat semen. Annual Meeting for the American Society of Animal Science, June 25-27, 2003, Phoenix, Arizona.
ARS News Articles
Last Modified: 5/2/2016
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