Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 5/21/2014
Publication Date: 12/1/2014
Citation: Purdy, P.H., Graham, J.K. 2014. Membrane modification strategies for cryopreservation. In: Wolkers, W.F., Oldenhof, H., editors. Cryopreservation and Freeze-Drying Protocls. Third Edition. The Lab Protocol Series Methods in Molecular Biology. Book Chapter. New York: Springer. p. 337-342. Interpretive Summary:
Technical Abstract: Cell membranes can be modified using cyclodextrins loaded with lipids or unilamellar liposomes. Lipid choice can greatly influence the organization of the targeted membrane and result in a cell that is more capable of surviving cryopreservation due to altered membrane phase transition properties or membrane reorganization that may alter the normal physiologic processes of the treated cell. The protocols described here will explain the preparation of the cyclodextrins and liposomes, impact of the amount and type of lipids, and general principles for treating cells using either of these technologies.