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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Agricultural Genetic Resources Preservation Research » Research » Publications at this Location » Publication #380902

Research Project: National Animal Germplasm Program

Location: Agricultural Genetic Resources Preservation Research

Title: Section 6: Collection and cryopreservation of germplasm and tissues

item BLESBOIS, ELISABETH - French National Institute For Agricultural Research
item Purdy, Phil
item SANTIAGO MORENO, JULIÁN - Instituto Nacional De Investigacion Y Technologia Agraria Y Alimentaria
item LIPTÓI, KRISZTINA - Genetics & Ivf Institute
item Rajamohan, Arun
item DE SOUZA-FABJAN, JOANNA - Fluminense Federal University(UFF)
item SOMFAI, TAMÁS - National Agriculture And Food Research Organization (NARO), Agricultrual Research Center
item PARNPAI, RANGSUN - Suranaree University Of Technology
item MCGREW, MICHAEL - Roslin Institute
item MERMILLOD, PASCAL - French National Institute For Agricultural Research
item BAILEY, JANICE - Quebec Research Fund

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 9/3/2021
Publication Date: 1/13/2023
Citation: Blesbois, E., Purdy, P.H., Santiago Moreno, J., Liptói, K., Rajamohan, A., De Souza-Fabjan, J., Somfai, T., Parnpai, R., McGrew, M.J., Mermillod, P., Bailey, J. 2023. Section 6: Collection and cryopreservation of germplasm and tissues. In: Boes, J., Boettcher, P., Honkatukia, M., editors. Innovations in Cryoconservation of Animal Genetic Resources - Practical Guide. FAO Animal and Health Guidelines, No. 33. Rome, Italy. p. 105-157.

Interpretive Summary: n/a

Technical Abstract: The development of animal germ plasm collections is an important tool in the management of animal genetic diversity. Animal germ plasm cryobanking, however, is a challenging process since the cryopreserved germ plasm must recover, after the cryopreservation process, all its specific functional capacity to generate live, healthy offspring. The cryopreservation process is not a biological process and because of this most cells/tissues need specific adaptations that are unique to the type of germ plasm and, in some instances, the animal species. In addition to the freezing-thawing process itself, the upstream methods of sampling and fertility treatments, and the downstream methods of utilization all hold their own challenges. Consequently, this chapter describes the different types of germ plasm cryopreservation methods available for agricultural species, includes descriptions of best practices for their use, and an overview of methods to assess germ plasm quality.