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item SPILLER, S.
item STERNES, K.
item Purdy, Phil
item Blackburn, Harvey
item DZAKUMA, J.

Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2004
Publication Date: 7/25/2004
Citation: Realivasquez, R., Ericsson, S.A., Spiller, S.F., Campbell, W.T., Sternes, K.L., Purdy, P.H., Blackburn, H.D., Dzakuma, J.M. 2004. Spanish buck ss-b inhibin/activin (INHBB) microsatellite polymorphisms. American Society of Animal Science/American Society of Dairy Science/Poultry Science Association Annual Meeting, July 25-29, 2004, St. Louis, Missouri. Journal of Animal Science Supplement 2004. 82(Suppl 1): 377-378.

Interpretive Summary: DNA markers such as b -B inhibin/activin microsatellite polymorphisms may be useful in selecting for fertility potential. Therefore, this study was performed to determine if they are present in Spanish buck DNA. Subunits of INHBB can dimerize to form inhibin or can hetero-dimerize with INHBA or with another INHBB subunit to create activin which impede or stimulate, respectively, spermatogenesis through FSH regulation. Distinctive patterns in the DNA demonstrated that variation among males exists. These findings may imply genetic diversity or fertility potential and could therefore be used for selection strategies.

Technical Abstract: DNA markers could be useful in selecting for potential spermatozoal fertility. The purpose of this study was to determine if b -B inhibin/activin microsatellite polymorphisms were present in Spanish buck DNA. An a (INHA) subunit and a b -B (INHBB) subunit can dimerize to form inhibin. The b -B subunit can hetero-dimerize with a b -A (INHBA) or with another b -B subunit to create activin. Inhibins impede and activins stimulate spermatogenesis through FSH regulation. Two primer sets designed for human INHBB microsatellite polymorphisms, polymerase chain reaction and electrophoresis were utilized to examine purified DNA from 23 Spanish bucks. The first primer set (forward AAACAGCAAGACCCTGACTC, reverse TTAGCGTGCC-ATCCTCAT) showed distinctive bands located at 284 base pairs (bp) (1 hd), 282 bp (2 hd), 280 bp (14 hd), 278 bp (1 hd), or no detectable bands (5 hd). The second primer set (forward AACTCCATCCCG GTGC, reverse ATGTGTGCCTGTGGGTTTAG) also showed distinctive bands that were located at 248 bp (6 hd) and 246 bp (7 hd) or no detectable bands (10 hd). Significant deviation from the Hardy-Weinberg equilibrium was observed for both primer sets due to excess homozygosity. These potential polymorphic bands may imply genetic diversity and could be used for selection strategies. Additional studies will be needed to identify specific QTL. Key words: Inhibin, Activin, INHBB