Submitted to: Lipids
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/14/1998
Publication Date: N/A
Interpretive Summary: When plants are attacked by pests, a wide array of biochemical processes may occur. For example, when insects, fungi, or bacteria attack, many plants produce a chemical called traumatin, a chemical which facilitates repair of a wound. Traumatin-like compounds are widely studied due to their importance in resistance to pests. In this work, a new metabolite of traumatin, 9-hydroxytraumatin, was found in soybeans and alfalfa. It is also likely that, in addition to resistance, the new compound has a physiological function that has not yet been defined. A structurally similar compound is known to help alleviate the effects of oxygen stress in animals. This present work will benefit scientists interested in these chemically mediated defense mechanisms of plants as well as the evolutionary development of the bioactive chemicals.
Technical Abstract: 9-Hydroxy-traumatin, 9-hydroxy-12-oxo-10E-dodecenoic acid, was isolated as a product of 13S-hydroperoxy-9Z,11E-octadecadienoic acid as catalyzed by enzyme preparations of both soybean and alfalfa seedlings. This suggested that traumatin, 12-oxo-9Z- dodecenoid acid, was being converted into 9-hydroxy-traumatin in an analogous manner to the previously identified enzymic conversion of 3Z-nonenal and 3Z-hexenal into 4-hydroxy-2E-nonenal and 4- hydroxy-2E-hexenal, respectively. Other metabolites of 13S-hydroperoxy- 9Z,11E-octadecadienoic acid were similar for both soybean and alfalfa seedlings' preparations and are briefly described.