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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Publications at this Location » Publication #89362

Title: DEVELOPMENT AND APPLICATION OF FREE ZONE CAPILLARY ELECTROPHORESIS FOR THE SEPARATION OF CEREAL PROTEINS

Author
item BEAN, SCOTT
item Bietz, Jerold
item Lookhart, George

Submitted to: Seminars in Food Analysis
Publication Type: Review Article
Publication Acceptance Date: 7/14/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary: Review paper - no interpretive summary required.

Technical Abstract: Capillary electrophoresis (CE) is a relatively new method that has improved the ability to separate and characterize cereal proteins dramatically. Commercial CE instruments have only been available since 1990. Several modes of separation are possible but the simplest and perhaps the most commonly used is free zone capillary electrophoresis (FZCE). In FZCE, the proteins are injected into a capillary filled with buffer and separated, primarily, on the basis of differences in charge density. This paper reviews the development of FZCE methods applied to wheat, rice, and oat storage proteins. The changes in capillary inner diameter size, buffer types and additives have played a major part in improved separations. FZCE is clearly a valuable addition to protein characterization methods.