Submitted to: Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/27/1997
Publication Date: N/A
Technical Abstract: HS were isolated from a grassland soil using, in sequence, water, neutral pyrophosphate (Pyro), Pyro pH 10.6, Pyro + sodium hydroxide, and fractionated using a resin series in tandem. The fractions were relatively homogeneous with regard to charge density. The resin in tandem technique was used to isolate the HS from the drainage waters. NMR and FTIR spectroscopy, elemental, sugar (S), and amino acid (AA) analyses, titration, delta **13C and delta **15N, and chemical degradation data have shown similarities and differences between the samples. The similarities between the fractions from water and neutral Pyro reflect the levels of humification, and the differences in the S and AA contents suggest that hydrolysis of saccharides and peptides allows the soil HS to become solubilized in the soil solution. Fractions isolated in alkaline conditions were least humidified.