Submitted to: Phytochemical Society of North America Meeting and Newsletter
Publication Type: Abstract Only
Publication Acceptance Date: 8/16/1995
Publication Date: N/A
Technical Abstract: Esters of sterols and ferulic or p-coumaric acids are found, among others, in seeds of corn, wheat, rye, triticale and rice. Because the cinnamic acid derivative (CAD) moiety can occur as both cis and trans isomers, can be esterified to range of plant sterols and at least two CADs, HPLC separation of this group is challenging. Separation of the trans forms of more than 16 of these compounds by HPLC has been accomplished using acetonitrile:n-butanol:acetic acid:water (94:3:2:1) with a reverse phase C18 column. And retention times, relative to ergosterol and cholesteryl cinnamate, have been derived. Sitosteryl, sitostanyl, stigmasteryl, campesteryl, campestanyl and trans7-sitosteryl and trans7- campesteryl ferulates, have been identified in corn bran. The ferulates of cycloartenol, 24-methylene-cycloartenol, cyclobranol, sitosterol, sitostanol, campesterol and campestanol have been identified in rice bran and y-oryzanol. Use of a diol column allows fast screening of crude hexane extracts for this group of compounds and has allowed the identification of these compounds in Tripsacum dactyloides, Andropogon gerardi, Bothriochloa bladhii, and B. ischaemum; indicating a wider distribution for this group of lipids than previously reported.