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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Environmental Microbial & Food Safety Laboratory » Research » Publications at this Location » Publication #401295

Research Project: Evaluation of Genetic and Management Factors to Reduce Foodborne Pathogens and Antimicrobial Resistance in Dairy Cattle

Location: Environmental Microbial & Food Safety Laboratory

Title: Disinfection of Escherichia coli using the contamination sanitization inspection and disinfection (CSI-D) system

item SANDERS, JENNIFER MCCOY - Chapman University
item ALACOM, VANESSA - Chapman University
item MARQUIS, GRACE - Chapman University
item TABB, AMANDA - Chapman University
item Van Kessel, Jo Ann
item Sonnier, Jakeitha - Jackie
item Haley, Bradd
item BAEK, INSUCK - US Department Of Agriculture (USDA)
item Qin, Jianwei - Tony Qin
item Kim, Moon
item VASEFI, FARTASH - Safetyspect Inc
item SOKOLOV, STANISLAV - Safetyspect Inc
item HELLBERG, ROSALEE - Chapman University

Submitted to: Proceedings of SPIE
Publication Type: Proceedings
Publication Acceptance Date: 5/4/2023
Publication Date: 6/13/2023
Citation: Sanders, J., Alacom, V., Marquis, G., Tabb, A., Van Kessel, J.S., Sonnier, J.L., Haley, B.J., Baek, I., Qin, J., Kim, M.S., Vasefi, F., Sokolov, S., Hellberg, R. 2023. Disinfection of Escherichia coli using the contamination sanitization inspection and disinfection (CSI-D) system. Proceedings of SPIE. 125450F.

Interpretive Summary:

Technical Abstract: Escherichia coli and Salmonella enterica are major causes of gastrointestinal disease nationally. The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a handheld fluorescence-based imaging device designed to disinfect food contact surfaces using ultraviolet-C (UVC) illumination. This study aimed to determine the optimal parameters for the disinfection of E. coli and S. enterica using the CSI-D system. Generic E. coli and S. enterica serotypes Enteritidis, Newport, Typhimurium, and Javiana were grown on selective media, followed by transfer to Luria Bertani broth. After overnight incubation, the cultures were diluted and spread-plated on L-agar. The plates were exposed to high-intensity (10 mW/cm2) or low-intensity (5 mW/cm2) UVC for 1 s, 3 s, or 5 s. Exposed and control plates were incubated at room temperature for 2-3 h, then overnight at 37°C. Colonies were counted and compared between control and exposed plates. Three trials were conducted on separate days. The average of the trials showed that exposure times of 3-5 s at either intensity (high: 10 mW/cm2 or low: 5 mW/cm2) resulted in effective and consistent destruction of E. coli and S. enterica. The minimum reduction at 3 s and 5 s exposure for both intensities was 99.7-100% for E. coli and 90.9-100% for S. enterica. The 1 s exposure time showed inconsistent results, with a survival rate of 0.23-9.12% for E. coli and 0.00-61.48% for S. enterica. The results of this study show that exposure to UVC for at least 3 s is required to achieve consistent disinfection of 90.9-100% for generic E. coli and S. enterica Enteritidis, Newport, Typhimurium, and Javiana.