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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #400935

Research Project: Characterization of Quality and Marketability of Western U.S. Wheat Genotypes and Phenotypes

Location: Wheat Health, Genetics, and Quality Research

Title: Proteomics profiling of pea seed development in high protein line

item Daba, Sintayehu
item UMA, ARYAL - Purdue University
item Kiszonas, Alecia
item McGee, Rebecca

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/4/2023
Publication Date: 2/1/2023
Citation: Daba, S.D., Uma, A., Kiszonas, A., Mcgee, R.J. 2023. Proteomics profiling of pea seed development in high protein line. Plant and Animal Genome Conference. PO0921.

Interpretive Summary: N/A

Technical Abstract: Peas are primarily bred for high protein content, which is then used in a variety of food products, including meat substitutes and protein drinks. Seed development is a critical stage in the crop life cycle as it determines seed composition. Assessing the changes in protein profiles during the seed development period is crucial to understand variations in seed compositions. The purpose of this preliminary study is to investigate protein profiles in pea during the seed development period, and it considered two time points (15 days after anthesis (15DAA) vs maturity) in the high protein cultivar 'Cameor.' Matured seed of 'Cameor' had an average protein content of 26.7%, with a range of 26.3% to 27.1%. Proteomic analysis of the two-time points led to the identification of 812 proteins. Applying two criteria (fold change = 2.5 and p = 0.001), 22 proteins were significantly abundant at 15DAA, and another 21 proteins were significantly abundant at maturity. Seed biotin-containing protein, two convicillin, and srpin-ZX-like protein were among the proteins that were abundant at maturity. Vanillin aminotransferase and beta-amylase are among the proteins found in abundance at 15DAA. This research is part of a larger project that includes three lines (low and high protein lines, as well as one wrinkle type) evaluated at five different time points during seed development.