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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Sustainable Biofuels and Co-products Research » Research » Publications at this Location » Publication #394907

Research Project: Commercial Products from Lipids and Fibers

Location: Sustainable Biofuels and Co-products Research

Title: Draft genome sequences of the sophorolipid-producing yeast pseudohyphozyma bogoriensis ATCC 18809

item Msanne, Joseph
item Shao, Jonathan
item Ashby, Richard - Rick
item CAMPOS, PHILIP - Oak Ridge Institute For Science And Education (ORISE)
item Liu, Yanhong
item SOLAIMAN, DANIEL - Retired ARS Employee

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/10/2022
Publication Date: 11/30/2022
Citation: Msanne, J.N., Shao, J.Y., Ashby, R.D., Campos, P., Liu, Y., Solaiman, D. 2022. Draft genome sequences of the sophorolipid-producing yeast pseudohyphozyma bogoriensis ATCC 18809. Microbiology Resource Announcements.

Interpretive Summary: The yeast strain Pseudohyphozyma bogoriensis is capable of producing considerable amounts of high-value surface-active molecules (glycolipid biosurfactants, more specifically known as sophorolipids) which have large-scale application potential in the detergent and cosmetic industries. Understanding the DNA genome sequence contained within this yeast strain would offer vital knowledge of the genetic regulations that govern the synthesis of these industrially relevant molecules. This will help make the synthetic process more economical through the development of genetic engineering systems and, coupled with the application of low-value agricultural by-products or other cheap substrates, will provide essential information necessary to further reduce production costs and broaden the application base for these molecules.

Technical Abstract: The aerobic yeast Pseudohyphozyma (formerly Rhodotorula) bogoriensis is gaining attention as a microbial source for the production and secretion of a unique, high-value extracellular glycolipid (e.g., sophorolipid) with numerous possibilities for industrial applications. Here, we report on the genomic sequences of P. bogoriensis ATCC 18809 that would help improve the understanding of relevant metabolic pathways involved in sophorolipid biosynthesis and allow the development of more specific genome manipulation systems. Sequencing was performed using the PacBio Sequel System and the 26 Mbp genome has a GC content of 57% and encodes for 7,818 predicted proteins.