Location: Commodity Utilization ResearchTitle: Cottonseed extracts regulate cell viability and gene expression in human colon cancer cells
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/6/2021
Publication Date: N/A
Technical Abstract: Plant extracts have been used as medicinal remedy for various diseases. The objective of this study was to survey the effect of cottonseed extracts on cell viability and gene expression in human colon cancer cells. COLO 225 cells were treated with ethanol extracts from glanded and glandless cottonseed followed by MTT and qPCR assays. Cottonseed extracts showed minor effects on cell viability. qPCR assay analyzed 55 mRNA levels involved in several pathways including DGAT, GLUT, TTP, IL, gossypol-regulated genes and TTP-mediated mRNAs. BCL2 mRNA was selected as the internal reference for qPCR assay due to its stability among the 55 mRNAs analyzed. qPCR data showed minor effects of ethanol extracts from glanded seed coat and kernel and glandless seed coat on mRNA levels in the cells. However, glandless seed kernel extract significantly reduced mRNA levels of a number of genes involved in glucose transport, lipid biosynthesis and inflammation. The inhibitory effects of glandless kernel extract on gene expression may provide a useful opportunity for improving healthcare associated with colon cancer since it is safe to use without toxic gossypol contamination. This in turn may provide the potential of increasing cottonseed value by using ethanol extracts as a health intervention agent.