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Research Project: Gene Discovery and Crop Design for Current and New Rice Management Practices and Market Opportunities

Location: Dale Bumpers National Rice Research Center

Title: Curating the USDA-ARS rice germplasm collection: efficient accession management and characterization through phenotyping and genotyping

item Huggins, Trevis
item McClung, Anna
item Edwards, Jeremy
item Jia, Melissa
item Bockelman, Harold
item ALI, LIAKAT - University Of Arkansas
item Eizenga, Georgia

Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/4/2019
Publication Date: 1/6/2021
Citation: Huggins, T.D., McClung, A.M., Edwards, J., Jia, M.H., Bockelman, H.E., Ali, L.M., Eizenga, G.C. 2021. Curating the USDA-ARS rice germplasm collection: efficient accession management and characterization through phenotyping and genotyping. Proceedings of 38th Rice Technical Working Group Meeting, February 24-27, 2020, Orange Beach, Alabama. p 76. Electronic Publication.

Interpretive Summary:

Technical Abstract: Genebanks are an important source of genetic diversity for food crops world-wide and offer valuable information that can be used by plant breeders to improve agricultural productivity and nutritional quality. The National Small Grains Collection (NSGC) was established for this purpose. Currently the rice portion of the collection, henceforth referred to as “Rice-NSGC”, maintains 19,031 accessions of cultivated Asian rice (Oryza sativa L.), 193 African cultivated rice (O. glaberrima Steud.) and 54 Oryza wild species. Challenges facing genebank management include providing sufficient and accurate information to facilitate searching the collection, dealing with redundant accessions, seed mixtures, mis-identified accessions, and gaps in diversity, as well as lack of resources to thoroughly characterize the collection. To partially address these issues, a random set of 1,993 Rice-NSGC accessions, henceforth called the “2K set”, were phenotypically characterized for two phenological traits, plant height, 13 morphological traits, six grain quality traits, four production traits, resistance to three diseases, and two stress related traits and were genotyped with 11 fingerprint markers (FPM), one subspecies marker, and 14 trait specific markers (TSM). TSM were used to validate phenotypic data for fragrance, pericarp color, blast disease resistance, leaf and hull pubescence, apparent amylose content, starch pasting properties and gelatinization temperature, and plant height. Utilizing these markers, accessions were classified by species, O. sativa or O. glaberrima; subspecies, indica or japonica; subpopulation, aromatic, indica, aus, temperate or tropical japonica; and often by cultivar.