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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #375997

Research Project: Increasing the Value of Cottonseed

Location: Commodity Utilization Research

Title: Effect of time and storage condition on the performance of an electrochemical immunosensor for Salmonella

item BRITO, FRANCISCA - Universidad De Sao Paulo
item BEZERRA, LORENA - Universidade Estadual Do Ceara
item FIGUEIREDO, EVANIA - Universidade Estadual Do Ceara
item MELO, AIRIS - Universidad De Sao Paulo
item ALVES, CARLUCIO - Universidad De Sao Paulo
item Cheng, Huai
item Biswas, Atanu

Submitted to: Food Science and Technology (Campinas)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/8/2021
Publication Date: 3/24/2022
Citation: Brito, F.A.E., Furtado, R.F., Bezerra, L.C.R., Figueiredo, E.A.T., Melo, A.M.A., Alves, C.R., Cheng, H.N., Biswas, A. 2022. Effect of time and storage condition on the performance of an electrochemical immunosensor for Salmonella. Food Science and Technology (Campinas). 42:e91621.

Interpretive Summary: A major issue with food safety is Salmonella contamination, and a rapid, reliable, and specific testing method for Salmonella is needed by the food industry. Electrochemical biosensors are available in the market and can meet all these requirements, but very few studies have been done on the effects of the storage medium on the sensor’s stability and effectiveness. In this work a stabilizer storage solution has been formulated that can extend the shelf life of the immunosensor up to 90 days. This work enables the immunosensor to be used effectively in industry and provides the opportunity for further improvements in the future.

Technical Abstract: Electrochemical immunosensors are useful for the detection of Salmonella contamination in food. Yet, the stability of the immunosensors during storage has been scarcely studied despite its importance with respect to practical usage and market acceptance. In this study, a stabilizer storage solution was proposed for immunosensors for Salmonella detection. The immunosensors were stored for 15, 30, 60, 90 and 120 days at refrigerated (4 ºC) and room temperature (25 ºC) in the proposed formulation. Their performance was compared to those stored in a commercial stabilizer solution used for immunoglobulin. There was a statistical difference (p<0.05) between the two stabilizer solutions and the two temperatures. The proposed storage formulation was shown to maintain the performance of the immunosensor at 4 ºC after 90 days, enough to meet the industry's needs.