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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #370414

Research Project: New Microbial and Plant-Based Agents for Mosquito Control

Location: Crop Bioprotection Research

Title: Effect of life stage and pesticide exposure on the gut microbiota of Aedes albopictus and Culex pipiens L

Author
item JUMA, ELIJAH - University Of Illinois
item ALLAN, BRIAN - University Of Illinois
item KIM, CHANG-HYUN - University Of Illinois
item STONE, CHRISTOPHER - University Of Illinois
item Dunlap, Christopher
item Muturi, Ephantus (juma)

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/11/2020
Publication Date: 6/11/2020
Citation: Juma, E.O., Allan, B.F., Kim, C.-H., Stone, C., Dunlap, C., Muturi, E.J. 2020. Effect of life stage and pesticide exposure on the gut microbiota of Aedes albopictus and Culex pipiens L. Scientific Reports. 10:9489. https://doi.org/10.1038/s41598-020-66452-5.
DOI: https://doi.org/10.1038/s41598-020-66452-5

Interpretive Summary: Mosquitoes develop in a variety of aquatic habitats that are often exposed to pesticides via agricultural runoff or targeted vector control. The mosquito larvae thriving in these aquatic habitats feed on microbial communities associated with decaying organic matter. Some of these microbes colonize the mosquito gut and contribute important roles in mosquito biology. However, it remains unclear how these microbial communities respond to short term exposure to pesticides. This study used the Asian tiger mosquito and the northern house mosquito to investigate how exposure of mosquito larvae to sub-lethal concentrations of three common pesticides – atrazine, permethrin, and malathion affects the microbial communities that inhabit the mosquito gut. Pesticide exposure had significant effect on gut bacterial richness but not community composition, with the highest bacterial richness occurring in larval samples exposed to malathion and permethrin treatments. The bacterial community composition of larval and adult mosquito samples and water from their larval habitats was also significantly different. These findings suggest that the stage of development has a stronger impact on mosquito gut microbiota relative to larval exposure to sub-lethal pesticide concentrations.

Technical Abstract: Pesticide contaminants are common in aquatic environments and can modify microbial community composition and diversity. However, their role in shaping the microbiota of invertebrates living in these environments, such as mosquito larvae, is not well understood. We used microcosms to investigate how larval exposure to sub-lethal concentrations of three common pesticides – atrazine, permethrin, and malathion – affects the gut microbiota of Aedes albopictus and Culex pipiens mosquitoes. Illumina MiSeq sequencing of the V3-V4 hypervariable regions of the 16S rRNA gene was used to characterize the microbial communities of larval and adult stages of the two mosquito species and the water samples from microcosms treated with each of the pesticides, separately. Bacterial community composition differed according to sample type (larval stage vs. adult stage vs. water sample), but not by pesticide treatment. Bacterial OTU richness was highest in larval samples receiving malathion and permethrin treatments, intermediate in the water and larval samples from the atrazine treatment, and lowest in adult samples irrespective of the treatment. This study improves our current understanding of the effects of sublethal pesticide exposure on the mosquito gut microbial composition and diversity and provides a basis for future studies investigating how pesticides interact with mosquito gut microbiota and the potential implications for transmission of mosquito-borne pathogens.