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ARS Home » Southeast Area » Stuttgart, Arkansas » Harry K. Dupree Stuttgart National Aquaculture Research Cntr » Research » Publications at this Location » Publication #367133

Research Project: The Role of Mucosal Surfaces and Microflora in Immunity and Disease Prevention

Location: Harry K. Dupree Stuttgart National Aquaculture Research Cntr

Title: Gene expression profiling of catfish after treatment with copper sulfate and exposure to Flavobacterium columnare

item Abernathy, Jason
item Lange, Miles
item Farmer, Bradley
item Beck, Benjamin
item Straus, David - Dave

Submitted to: Genbank
Publication Type: Database / Dataset
Publication Acceptance Date: 8/22/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Catfish farming remains number one among U.S. aquaculture production. Flavobacterium columnare, the causative agent of columnaris disease, produces substantial mortality during the production of freshwater farmed finfish species. F. columnare is ubiquitous in the aquatic environment, and outbreaks of disease are often triggered during the spring and summer months of the catfish production cycle. As food fish production continues to increase, the frequency of columnaris disease will only continue to rise within the aquaculture industry and thus new preventative measures will be required. Copper sulfate is the most common algaecide used in aquaculture. Copper sulfate has also been suggested as a therapeutant for columnaris disease, as we have previously discovered copper sulfate treatment effects the survivability and gill histology of channel catfish during an infection with F. columnare. Therefore to evaluate the functional effects of treatment with copper sulfate on columnaris disease, we performed RNA sequencing on gill samples from control and copper sulfate-treated catfish either immediately (more susceptible) or 24 hours after (more resistant) copper treatment followed by a challenge with F. columnare. We evaluated the differential gene expression patterns over the time-course of infection between groups with a focus on identifying functional differences in immune responsiveness.