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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Meat Safety & Quality Research » Research » Publications at this Location » Publication #366257

Research Project: Genomic and Metagenomic Differences in Foodborne Pathogens and Determination of Ecological Niches and Reservoirs

Location: Meat Safety & Quality Research

Title: Complete genome sequences of four Salmonella enterica strains (including those of serotypes Montevideo, Mbandaka, and Lubbock) isolated from peripheral lymph nodes of healthy cattle

Author
item BUGAREL, MARIE - Texas Tech University
item COOK, PETER - Centers For Disease Control And Prevention (CDCP) - United States
item DEN BAKKER, HENK - University Of Georgia
item Harhay, Dayna
item NIGHTINGALE, KENDRA - Texas Tech University
item LONERAGAN, GUY - Texas Tech University

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/3/2018
Publication Date: 1/10/2019
Citation: Bugarel, M., Cook, P.W., Den Bakker, H.C., Harhay, D.M., Nightingale, K.K., Loneragan, G.H. 2019. Complete genome sequences of four Salmonella enterica strains (including those of serotypes Montevideo, Mbandaka, and Lubbock) isolated from peripheral lymph nodes of healthy cattle. Microbiology Resource Announcements. 8(2):e01450-18. https://doi.org/10.1128/MRA.01450-18.
DOI: https://doi.org/10.1128/MRA.01450-18

Interpretive Summary: Salmonella enterica is a foodborne pathogen that inhabits and colonizes a large variety of hosts and environments. Recent studies have highlighted the presence of Salmonella in the lymph nodes of healthy cattle at harvest. If incorporated with beef trim to produce ground beef, these tissues may become a source of beef contamination. A recently characterized Salmonella strain, commonly isolated from healthy cattle in the southern United States, is Salmonella Lubbock. This novel strain has been isolated from lymph nodes as well as from other beef tissues, and most likely emerged as the result of a recombination event between Salmonella Montevideo and Salmonella Mbandaka ancestors. Here we report the complete closed genome sequences of four strains, including two Salmonella Lubbock, one Salmonella Montevideo and one Salmonella Mbandaka. These sequence data lay the groundwork for determining the possible genomic evolutionary events that resulted in the emergence of the Salmonella Lubbock strain.

Technical Abstract: Salmonella enterica serotype Lubbock emerged most likely from a Salmonella enterica serotype Mbandaka ancestor that acquired by recombination the fliC operon from Salmonella enterica serotype Montevideo. Here, we report the complete genome sequence of two S. Lubbock, one S. Montevideo, and one S. Mbandaka strain isolated from bovine lymph nodes.