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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #366018

Research Project: Improving Feed Efficiency and Environmental Sustainability of Dairy Cattle through Genomics and Novel Technologies

Location: Animal Genomics and Improvement Laboratory

Title: Epigenomic profiling of histone marks and CTCF binding sites in bovine rumen epithelial primary cells before and after butyrate treatment

Author
item KANG, XIAOLONG - Ningxia University
item LIU, SHULI - China Agricultural University
item FANG, LINGZHAO - University Of Edinburgh
item LIN, SHUDAI - South China Agricultural University
item LIU, MEI - Northwest Agriculture And Forestry University
item Baldwin, Ransom - Randy
item Liu, Ge - George
item Li, Congjun

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/4/2019
Publication Date: 2/1/2020
Citation: Kang, X., Liu, S., Fang, L., Lin, S., Liu, M., Baldwin, R.L., Liu, G., Li, C. 2020. Epigenomic profiling of histone marks and CTCF binding sites in bovine rumen epithelial primary cells before and after butyrate treatment. Data in Brief. 28:104983. https://doi.org/10.1016/j.dib.2019.104983.
DOI: https://doi.org/10.1016/j.dib.2019.104983

Interpretive Summary: We generated and deposited the genome-wide datasets of transcript factor binding sites of CTCF CCCTC-binding factor, histone methylation and histone acetylation from bovine rumen epithelial primary cells before and after butyrate treatment. In this data descriptor, we provide detailed information on experiment design, data generation, data quality assessment and guideline for data re-use. Our data will be a valuable resource for systematic annotation of regulatory elements in cattle and the functionally biological role of butyrate in the epigenetic modifications in bovine, as well as for the nutritional regulation and metabolism study of farm animal and human.

Technical Abstract: Discovering the regulatory elements of genomes in livestock is essential for our understanding of livestock’s basic biology and genomic improvement programs. Previous studies showed butyrate mediates epigenetic modifications of bovine cells. To explore the bovine functional genomic elements and the vital roles of butyrate on the epigenetic modifications of bovine genomic activities, we generated and deposited the genome-wide datasets of transcript factor binding sites of CTCF (CCCTC-binding factor, insulator binding protein), histone methylation (H3H27me3, H3K4me1, H3K4me3) and histone acetylation (H3K27ac) from bovine rumen epithelial primary cells (REPC) before and after butyrate treatment. In this data descriptor, we provide detailed information on experiment design, data generation, data quality assessment and guideline for data re-use. Our data will be a valuable resource for systematic annotation of regulatory elements in cattle and the functionally biological role of butyrate in the epigenetic modifications in bovine, as well as for the nutritional regulation and metabolism study of farm animal and human.