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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #364610

Research Project: Use of Microorganisms to Manage Weeds and Insect Pests in Turf and Agricultural Systems

Location: Crop Bioprotection Research

Title: Prevotella brunnea sp. nov., isolated from a wound of a patient

Author
item BUHL, MICHAEL - Tübingen University Hospital
item Dunlap, Christopher
item MARSCHAL, MATTHIAS - Tübingen University Hospital

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2019
Publication Date: 12/1/2019
Citation: Buhl, M., Dunlap, C., Marschal, M. 2019. Prevotella brunnea sp. nov., isolated from a wound of a patient. International Journal of Systematic and Evolutionary Microbiology. 69:3933-3938. https://doi.org/10.1099/ijsem.0.003715.
DOI: https://doi.org/10.1099/ijsem.0.003715

Interpretive Summary: ARS researchers from Peoria, IL collaborated with scientists from Tübingen University Hospital in Tübingen, Germany to characterize a novel bacterium found in a hospital environment. This manuscript reports on the characterization of a new species of bacteria , Prevotella brunnea that grows under anaerobic conditions. This species may possess novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes.

Technical Abstract: A strain of an obligately anaerobic, Gram stain-negative rod-shaped bacterium is described by phenotypical, biochemical and genotypical characterization.This strain A2672T was isolated from a wound of a patient sampled during routine care at hospital. Phylogenetic analysis was based on full-length 16S rRNA gene sequence analysis and revealed the strain to belong to the genus Prevotella, but to be distant from known species, with the closest relationship to Prevotella corporis. The genomic DNA G+C content was 44.0 mol%. Strain A2672T was moderately saccharolytic and proteolytic. The most abundant cellular long-chain fatty acids were anteiso-C15:0 and iso-C15:0. In view of phenotypical, biochemical and genotypical characteristics as well as whole-genome sequence analysis, strain A2672T is considered to represent a novel species within the genus Prevotella, for which the name Prevotella brunnea sp. nov. is proposed. The type strain is A2672T (= DSM 108033T = CCOS 1231T).