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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #363183

Research Project: Wheat Quality, Functionality and Marketablility in the Western U.S.

Location: Wheat Health, Genetics, and Quality Research

Title: Registration of extra-hard kernel near-isogenic hexaploid wheat genetic stocks lacking puroindoline genes

item Morris, Craig
item Kiszonas, Alecia
item Peden, Gail

Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/15/2019
Publication Date: 3/6/2020
Publication URL:
Citation: Morris, C.F., Kiszonas, A., Peden, G.L. 2020. Registration of extra-hard kernel near-isogenic hexaploid wheat genetic stocks lacking puroindoline genes. Journal of Plant Registrations. 14(1):92-95.

Interpretive Summary: Kernel texture (‘hardness’) is a primary determinant of wheat (Triticum spp. L.) grain quality and utilization. In general, three major texture classes are recognized: soft and hard hexaploid (T. aestivum L.) and durum (T. turgidum ssp. durum [Desf. (Husn.)]). These three classes are directly associated with the presence/absence of the Hardness (Ha) locus, or its allelic state (‘soft’ or ‘hard’). The two puroindoline NILs were produced in the soft white spring (SWS) hexaploid wheat (T. aestivum L.) cv. Alpowa (PI 566595) via repeated back-crossing. Alpowa was the leading SWS wheat cultivar in Washington from 1997-2006, reaching 116,000 ha at its peak. These NILs extend a series of kernel texture/puroindoline allelic sequence NILs in Alpowa. Additional kernel texture NILs are available in winter, spring and waxy wheat backgrounds.

Technical Abstract: Kernel hardness (texture) is a key end-use quality trait of wheat (Triticum spp.). Two hexaploid wheat (T. aestivum L.) near-isogenic lines (NILs), WQL18RE5 and WQL18CS5, lack the puroindoline genes and thus have kernel texture phenotypes more similar to durum wheat (T. turgidum subsp. durum). These two genetic stocks were produced in cv. Alpowa soft white spring wheat (PI 566595), and comprise extensions of an on-going series of near-isogenic lines (NIL) in this soft wheat cultivar. Both NILs are BC7. WQL18RE5 was derived from a Chinese Spring (CS) disomic substitution line, CS (Red Egyptian 5D), whereas WQL18CS5 was derived from a CS deletion line (CS5DS del.-2) which lacks the distal portion of the short arm of chromosome 5D. These lines will be useful to study kernel texture and end-use quality in hexaploid wheat, and can serve as parents in developing cultivars with kernel texture commensurate with that of durum wheat.