Skip to main content
ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #361676

Research Project: Genetic and Environmental Factors Controlling Aflatoxin Biosynthesis

Location: Food and Feed Safety Research

Title: Developmental and metabolic processes impacted in Aspergillus flavus during maize kernel infection under various environmental conditions

item Gilbert, Matthew
item Mack, Brian
item MEDINA, ANGEL - Cranfield University
item GARCIA-CELA, ESTER - Cranfield University
item MAGAN, NARESH - Cranfield University
item Bhatnagar, Deepak

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/21/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Aspergillus flavus is an opportunistic fungus that can infect maize and other crops of agricultural importance. This fungus survives in soil and upon infecting crops has the potential to produce carcinogenic mycotoxins such as aflatoxin. Predicted changes in global temperatures, precipitation patterns and carbon dioxide levels are expected to impact the growth, virulence, and production of secondary metabolites in A. flavus. Here we report the results of transcriptomic, metabolic, and developmental analysis of A. flavus after various temperature, water activity, and carbon dioxide treatments during the maize-fungus interaction. RNA-sequencing of maize-fungus samples, incubated in situ and collected at several time points, were exposed to combinations of these environmental variables. MS-HPLC analysis was also conducted to identify the impact on the metabolome. Analysis of this data includes the computational determination of gene networks involved in the maize-host interaction, and how these networks shift under environmental stressors. Special emphasis is placed on the role carbon dioxide levels have in mediating the response to water activity levels and temperature.