Location: Healthy Processed Foods ResearchTitle: Improved chemical stability and antiproliferative activities of curcumin-loaded nanoparticles with a chitosan chlorogenic acid conjugate
|FAN, YUTING - Shenzhen University|
|YI, JIANG - Shenzhen University|
|Yokoyama, Wallace - Wally|
Submitted to: Journal of Agriculture and Food Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/20/2017
Publication Date: 11/20/2017
Citation: Fan, Y., Yi, J., Zhang, Y., Yokoyama, W.H. 2017. Improved chemical stability and antiproliferative activities of curcumin-loaded nanoparticles with a chitosan chlorogenic acid conjugate. Journal of Agriculture and Food Sciences. 65:10812-10819.
Interpretive Summary: Curcumin, the bioactive component of turmeric, has been shown to have many health promoting properties. However, curcumin is poorly soluble in both water and oil, and is also chemically unstable. Delivering curcumin in nanoparticles may increase bioavailability by increasing surface area. The study showed greater antiproliferative activity in a cancer cell model.
Technical Abstract: A chitosan (CS)-chlorogenic acid (CA) conjugate was successfully prepared through free-radical-induced protocols with a substitution of CA on CS of 103.5 mg/g. ATR-FTIR and 1 H NMR results validated the covalent conjugation of CA onto CS. XRD results indicated the decrease of crystallinity after CA conjugation. DPPH-scavenging activity and reducing power studies indicated that the CS-CA conjugate had stronger antioxidant activity than chitosan. The particle diameters of curcumin-loaded CS and CS-CA nanoparticles simultaneously formed by ionic gelling in the presence of tripolyphosphate (TPP) were less than 300 nm (243.6 and 256.5 nm, respectively), and zeta-potential values between 25 and 30 mV were obtained. TEM results showed that the nanoparticles were spherically shaped and homogeneously dispersed. Curcumin with the CS-CA conjugate showed better heat stability than with CA at both temperatures (25 and 95 °C). Curcumin release was inhibited by the CS-CA conjugate. The total release amount of curcumin from CS and CS-CA-conjugate nanoparticles were 70.5 and 61.7%, respectively. Curcumin release was inhibited by the CS-CA conjugate. The total release amount of curcumin from CS and CS-CA-conjugate nanoparticles were 70.5 and 61.7%, respectively. A methyl thiazolyl tetrazolium (MTT) assay showed that the antiproliferative activity of curcumin in CS-CA nanoparticles was remarkably higher than that in CS nanoparticles because of the higher chemical stability. The results suggest that CS-CA-based nanoparticles are promising candidates for the encapsulation and controlled release of hydrophobic, bioactive compounds and can improve these compounds’ chemical stabilities and anticancer activities.