Location: Crop Bioprotection ResearchTitle: Activity of a maize chitinase-like protein against maize insect pests
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/26/2018
Publication Date: 11/11/2018
Citation: Dowd, P.F., Naumann, T.A., Johnson, E.T., Price, N.P. 2018. Activity of a maize chitinase-like protein against maize insect pests. Meeting Abstract.
Technical Abstract: Maize resistance to insects and fungi is thought to be due to the contribution of a number of proteins and secondary metabolites. Identification of the responsible resistance genes can facilitate the development of maize lines suitable for sustainable production. A gene located in an ear rot fungus resistance quantitative trait locus had similarity to a chitinase and was cloned from an ear rot resistant inbred. The gene was expressed in Pichia pastoris expression system and purified. The protein did not have any detectible chitinase activity, and did not bind to a chitin column. However, it caused significant reduction in growth rates of fall armyworm when incorporated into pinto bean and maize leaf diets, and also enhanced antifungal activity of miconazole towards the maize ear rot pathogen Fusarium gramnearum.