Location: Crop Bioprotection ResearchTitle: Phylogenomic analysis of the Brevibacillus brevis clade: a proposal for three new Brevibacillus species, Brevibacillus fortis sp. nov., Brevibacillus porteri sp. nov. and Brevibacillus schisleri sp. nov.
Submitted to: Antonie Van Leeuwenhoek
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/17/2019
Publication Date: 1/29/2019
Citation: Johnson, E.T., Dunlap, C.A. 2019. Phylogenomic analysis of the Brevibacillus brevis clade: a proposal for three new Brevibacillus species, Brevibacillus fortis sp. nov., Brevibacillus porteri sp. nov. and Brevibacillus schisleri sp. nov. Antonie Van Leeuwenhoek. https://doi.org/10.1007/s10482-019-01232-4.
Interpretive Summary: We performed studies of the entire genetic material of several strains of bacteria that are publicly available from the ARS culture collection. This study describes 3 new species of bacteria, 2 of which secrete compounds that inhibit growth of a fungus pest of onions. Further research on these bacteria could lead to their development as an agricultural pest control product.
Technical Abstract: Screening of Brevibacillus species in the Northern Regional Research Laboratory collection identified 3 strains with antifungal activity. Subsequent genomic sequencing and phylogenomic analysis revealed that 2 of these strains (NRRL NRS-1210**T and NRRL B-41110**T) were novel species. Phylogenomic analysis of other closely related Brevibacillus strains also identified a novel clade, which included ATCC 35690**T, that should be recognized as a novel species. The 3 new species were subjected to a polyphasic characterization to confirm their taxonomic status. Cells of strains NRRL NRS-1210**T, NRRL B-41110**T and ATCC 35690**T were Gram staining positive, motile and formed tan colonies. All 3 strains were obligate aerobic mesophiles with a broad pH range for growth. The two most prominent fatty acids of the 3 strains were 15:0 iso and 15:0 anteiso. The DNA G+C contents of strains NRRL NRS-1210**T, NRRL B-41110**T and ATCC 35690**T were 47.2 mol%, 47.1 mol% and 47.3 mol%, respectively. Based on these characteristics, 3 novel species are proposed: Brevibacillus fortis sp. nov. (NRRL NRS-1210**T=DSM 9886**T=ATCC 51666**T), Brevibacillus roveniae sp. nov. (NRRL B-41110**T=KACC ###**T) and Brevibacillus schisleri sp. nov. (ATCC 35690**T=LMG 17055**T).