Location: Crop Bioprotection ResearchTitle: Lysinibacillus capsici PB300: from a single strain to a finished formulated Microbial Inoculant
|BURKETT-CADENA, M - Pathway Biologic|
|HUANG, P - Pathway Biologic|
|SASTOQUE, L - Pathway Biologic|
|CADENA, J - Pathway Biologic|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/17/2018
Publication Date: 6/17/2018
Citation: Burkett-Cadena, M., Huang, P., Sastoque, L., Cadena, J., Dunlap, C.A. 2018. Lysinibacillus capsici PB300: from a single strain to a finished formulated Microbial Inoculant. Meeting Abstract.
Technical Abstract: A gram positive, rod-shaped bacterium (PB300), isolated from the rhizosphere of a pepper plant, was identified to species and characterized for its capacity to promote plant growth. A draft genome of PB300 was completed and based upon the consensus of phylogenetic and phenotypic analyses, it was concluded that this strain represents a novel species within the genus Lysinibacillus, for which the name Lysinibacillus capsici sp. nov. is proposed. Characterization of PB300 for expression of PGPR traits using 5L bioreactors showed production of high levels of IAA (33 µg/mL in 18 hours). Plant growth promotion of PB300 was evaluated in greenhouse studies in apple, corn, and cabbage using either a liquid suspension of the spores or a wettable powder. In apple two applications of PB300 spore suspension at 1.0x10**7 CFU/mL, resulted in a significant increase in dry weight of shoots (30%) and roots (30%). Application of PB300 1.0x10**7 CFU/mL in furrow and again to the soil surface significantly promoted early growth of irrigated corn, and also significantly enhanced drought tolerance of corn at the V5 stage of growth. Applying PB300 at the three concentrations significantly promoted growth of cabbage when compared to the non-inoculated control. These results indicate that L. capsici PB300 represents a viable candidate for development as a microbial inoculant, stimulating progress of a wettable powder. The viability of the formulation was validated under lab conditions mimicking typical storage conditions (37 °C and moisture 70 % moisture) for 24 months. Results demonstrated that survival of PB300 is approximately 100% (>10**9 CFU/g) for a two-year storage period under suboptimal conditions. This work advances our knowledge on Lysinibacillus strains providing valuable information towards the use of novel species as active ingredients for Microbial inoculants highly demanded in the market in the current era. Microbial inoculants that are also preferred in the industry based not only on efficacy but also on convenience for storage, stability, and transportation.