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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Food Safety and Intervention Technologies Research » Research » Publications at this Location » Publication #349777

Research Project: The Role of Genotype in the Development and Validation of Growth Models and Intervention Technologies for Pathogenic Non-Shiga Toxigenic Escherichia coli Found in Foods

Location: Food Safety and Intervention Technologies Research

Title: Draft genome sequences of six neonatal meningitis-causing escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65)

Author
item Xu, Aixia - Oak Ridge Institute For Science And Education (ORISE)
item Johnson, James - Department Of Veterans Affairs
item Sheen, Shiowshuh
item Sommers, Christopher

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/16/2018
Publication Date: 4/19/2018
Citation: Xu, A., Johnson, J., Sheen, S., Sommers, C.H. 2018. Draft genome sequences of six neonatal meningitis-causing escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65). Genome Announcements. https://doi.org/10.1128/genomeA00091-18.
DOI: https://doi.org/10.1128/genomeA00091-18

Interpretive Summary: Extraintestinal Pathogenic Escherichia coli (ExPEC), including Neonatal Meningitis Causing E. coli (NMEC), are common contaminants in foods. Once ingested NMEC are able to colonize the gastrointestinal tract of the mother and during birth NMEC contaminated feces are sometimes transferred into the sterile mouth and nose of the newborn where they quickly multiply and cause meningitis. Genomics is now considered an integral part of microbial risk assessments for food safety. In this study we report the genomic DNA sequence of six clinical NMEC isolates which includes important characteristics such as their virulence factors and antibiotic resistances. This study data will allow improved risk assessments which will provide safer foods for consumers.

Technical Abstract: Neonatal meningitis Escherichia coli isolates (SP-4, SP-5, SP-13, SP-16, SP-46, and SP-65) were recovered from infants in the Netherlands from 1989 to 1997. Here, we report the draft genome sequences for these six E. coli isolates, which are currently being used to validate food safety processing technologies.